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  4. Recombinant Saccharomyces cerevisiae Meiotic recombination protein DMC1 (DMC1)

Recombinant Saccharomyces cerevisiae Meiotic recombination protein DMC1 (DMC1)

  • 中文名稱：
    釀酒酵母DMC1重組蛋白
  • 貨號：
    CSB-YP338672SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母DMC1重組蛋白
  • 貨號：
    CSB-EP338672SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母DMC1重組蛋白
  • 貨號：
    CSB-BP338672SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母DMC1重組蛋白
  • 貨號：
    CSB-MP338672SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    DMC1
  • Uniprot No.：
  • 別名：
    DMC1; ISC2; YER179WMeiotic recombination protein DMC1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-334
  • 氨基酸序列
    MSVTGTEIDS DTAKNILSVD ELQNYGINAS DLQKLKSGGI YTVNTVLSTT RRHLCKIKGL SEVKVEKIKE AAGKIIQVGF IPATVQLDIR QRVYSLSTGS KQLDSILGGG IMTMSITEVF GEFRCGKTQM SHTLCVTTQL PREMGGGEGK VAYIDTEGTF RPERIKQIAE GYELDPESCL ANVSYARALN SEHQMELVEQ LGEELSSGDY RLIVVDSIMA NFRVDYCGRG ELSERQQKLN QHLFKLNRLA EEFNVAVFLT NQVQSDPGAS ALFASADGRK PIGGHVLAHA SATRILLRKG RGDERVAKLQ DSPDMPEKEC VYVIGEKGIT DSSD
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Required for meiotic recombination, synaptonemal complex formation and cell cycle progression. Recruited to meiosis recombination hotspots by MEI5 and SAE3.
  • 基因功能參考文獻(xiàn)：
    1. Dmc1's dominance in promoting strand exchange between homologs involves repression of Rad51's strand-exchange activity. PMID: 24367271
    2. Negative staining electron microscopy and iterative helical real-space reconstruction revealed that the ScDmc1-ssDNA nucleoprotein filament harbors 6.5 protomers per turn. PMID: 23769192
    3. study provides biochemical evidence that Rad51 acts as an accessory factor with Mei5-Sae3 for Dmc1-mediated joint molecule formation during meiosis; Rad51 is a multifunctional protein that catalyzes recombination directly in mitosis and indirectly, via Dmc1, during meiosis PMID: 22955832
    4. Dmc1-Mei5-Sae3 complex is integrated onto Rad51 ensembles and plays both catalytic and structural roles in interhomolog recombination during meiosis. PMID: 15620352
    5. Dmc1 forms rings with an external diameter of 18 nm and a central cavity of 4 nm PMID: 15835894
    6. data support the view that the active form of Dmc1 protein is a helical filament rather than a ring and that calcium plays a significant role in regulating meiotic recombination PMID: 16204247
    7. MND1/HOP2 facilitatea DMC1-dependent interhomolog crossover formation in meiosis. PMID: 16581767
    8. Deletion of DMC1 abolished DNA rereplication, but additional deletion of RAD17 restored the original phenotype PMID: 19116279

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  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    RecA family, DMC1 subfamily
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YER179W

    STRING: 4932.YER179W