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  4. Recombinant Saccharomyces cerevisiae Regulator of Ty1 transposition protein 107 (RTT107), partial

Recombinant Saccharomyces cerevisiae Regulator of Ty1 transposition protein 107 (RTT107), partial

  • 中文名稱：
    釀酒酵母RTT107重組蛋白
  • 貨號：
    CSB-YP327899SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母RTT107重組蛋白
  • 貨號：
    CSB-EP327899SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母RTT107重組蛋白
  • 貨號：
    CSB-EP327899SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母RTT107重組蛋白
  • 貨號：
    CSB-BP327899SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母RTT107重組蛋白
  • 貨號：
    CSB-MP327899SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RTT107
  • Uniprot No.：
  • 別名：
    RTT107; ESC4; YHR154W; Regulator of Ty1 transposition protein 107; Establishes silent chromatin protein 4
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Required for resumption of chromosome replication after DNA damage, specifically in S phase. Is recruited to chromatin in the presence of RTT109 and RTT101 in response to stalled replication forks and acts as a scaffold during DNA repair.
  • 基因功能參考文獻：
    1. A key function of the Rtt107 BRCT domains for targeting of both itself and its interaction partners to DNA lesions was revealed. PMID: 26641499
    2. The DNA repair scaffold proteins Slx4 and Rtt107 utilize a minimal multi-BRCT-domain module for phosphatase-independent downregulation of DNA damage response signals in yeast. PMID: 25896509
    3. The C-terminal tandem BRCT repeats of Rtt107 interaction with gammaH2A is functionally important during DNA repair. PMID: 22262834
    4. a multifaceted functional relationship between Rtt107 and Dot1 in the DNA damage response and maintenance of genome integrity. PMID: 20810656
    5. identify Esc4p as an important new S-phase-specific target of monitors and participates in meiotic recombination Mec1p PMID: 14988729
    6. Slx4-dependent phosphorylation of Rtt107 by Mec1 is critical for replication restart after alkylation damage. PMID: 16267268
    7. Esc4 binds the Rad55/Rad56 heterodimer and forms subnuclear foci in response to DNA damage that stalls replication forks. PMID: 16569515
    8. Rtt107 is recruited to chromatin following DNA damage that stalls DNA replication forks. This recruitment depends on the acetyltransferase Rtt109 and the cullin Rtt101. Chromatin-bound Rtt107 is localized at or near stalled replication forks. PMID: 17978089

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  • 亞細胞定位：
    Nucleus. Note=Recruited to chromatin in response to replication fork stalling.
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YHR154W

    STRING: 4932.YHR154W