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  4. Recombinant Saccharomyces cerevisiae Translation initiation factor eIF-2B subunit alpha (GCN3)

Recombinant Saccharomyces cerevisiae Translation initiation factor eIF-2B subunit alpha (GCN3)

  • 中文名稱：
    釀酒酵母GCN3重組蛋白
  • 貨號：
    CSB-YP007514SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母GCN3重組蛋白
  • 貨號：
    CSB-EP007514SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母GCN3重組蛋白
  • 貨號：
    CSB-EP007514SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母GCN3重組蛋白
  • 貨號：
    CSB-BP007514SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母GCN3重組蛋白
  • 貨號：
    CSB-MP007514SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    GCN3
  • Uniprot No.：
  • 別名：
    GCN3; AAS2; TIF221; YKR026CTranslation initiation factor eIF-2B subunit alpha; GCD complex subunit GCN3; Guanine nucleotide exchange factor subunit GCN3; Transcriptional activator GCN3; eIF-2B GDP-GTP exchange factor subunit alpha
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區(qū)域：
    2-305
  • 氨基酸序列
    SEFNITETY LRFLEEDTEM TMPIAAIEAL VTLLRIKTPE TAAEMINTIK SSTEELIKSI PNSVSLRAGC DIFMRFVLRN LHLYGDWENC KQHLIENGQL FVSRAKKSRN KIAEIGVDFI ADDDIILVHG YSRAVFSLLN HAANKFIRFR CVVTESRPSK QGNQLYTLLE QKGIPVTLIV DSAVGAVIDK VDKVFVGAEG VAESGGIINL VGTYSVGVLA HNARKPFYVV TESHKFVRMF PLSSDDLPMA GPPLDFTRRT DDLEDALRGP TIDYTAQEYI TALITDLGVL TPSAVSEELI KMWYD
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Acts as a non-essential regulatory component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)-bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the translation of GCN4 in response to low amino acid, carbon, or purine availability, by suppressing the inhibitory effects of multiple uORFs present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. Modulation of GCN3 regulatory function in response to amino acid availability occurs post-translationally.
  • 基因功能參考文獻：
    1. Maintenance of inhibition required GCN-dependent hyperphosphorylation of eIF2alpha PMID: 15930127
  • 蛋白家族：
    EIF-2B alpha/beta/delta subunits family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YKR026C

    STRING: 4932.YKR026C