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  4. Recombinant Saccharomyces cerevisiae Vacuolar protein-sorting-associated protein 46 (DID2)

Recombinant Saccharomyces cerevisiae Vacuolar protein-sorting-associated protein 46 (DID2)

  • 中文名稱：
    釀酒酵母DID2重組蛋白
  • 貨號(hào)：
    CSB-YP304030SVG
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母DID2重組蛋白
  • 貨號(hào)：
    CSB-EP304030SVG
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母DID2重組蛋白
  • 貨號(hào)：
    CSB-EP304030SVG-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母DID2重組蛋白
  • 貨號(hào)：
    CSB-BP304030SVG
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母DID2重組蛋白
  • 貨號(hào)：
    CSB-MP304030SVG
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    DID2
  • Uniprot No.：
  • 別名：
    DID2; CHM1; FTI1; VPS46; YKR035W-AVacuolar protein-sorting-associated protein 46; Charged multivesicular body protein 1; DOA4-independent degradation protein 2; Fifty two inhibitor 1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-204
  • 氨基酸序列
    MSRNSAAGLE NTLFQLKFTS KQLQKQANKA SKEEKQETNK LKRALNENED ISRIYASNAI RKKNERLQLL KLASRVDSVA SRVQTAVTMR QVSASMGQVC KGMDKALQNM NLQQITMIMD KFEQQFEDLD TSVNVYEDMG VNSDAMLVDN DKVDELMSKV ADENGMELKQ SAKLDNVPEI KAKEVNVDDE KEDKLAQRLR ALRG
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Class E VPS protein implicated in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins will be targeted into the vacuole after fusion of the endosome with the vacuole. Probably acts as a peripherally associated component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruits late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. Regulates the membrane association of VPS4. Can stimulate VPS4 ATPase activity directly or via VTA1.
  • 基因功能參考文獻(xiàn)：
    1. the ESCRT regulator Did2 coordinates precise maturation of endosomes and thus provides the correct membrane identity for efficient endosomal long-distance transport. PMID: 28422978
    2. Cocrystallization of Ist1NTD with a Did2 fragment shows that Ist1 interacts with the Did2 C-terminal MIM1 (MIT-interacting motif 1) via a novel MIM-binding structural motif. PMID: 19477918
    3. We show that Did2 directs Vps4 activity to the dissociation of ESCRT-III but has no role in the dissociation of ESCRT-I or -II. PMID: 17130288
    4. A model in which the Ist1-Did2 and Vta1-Vps60 complexes independently modulate late steps in the multivesicular body-sorting pathway, is proposed. PMID: 18032584
  • 亞細(xì)胞定位：
    Endosome membrane; Peripheral membrane protein. Endomembrane system; Peripheral membrane protein. Note=Endosomal and other punctate structures.
  • 蛋白家族：
    SNF7 family
  • 數(shù)據(jù)庫(kù)鏈接：