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  4. Recombinant Saccharomyces cerevisiae tRNA dimethylallyltransferase, mitochondrial (MOD5)

Recombinant Saccharomyces cerevisiae tRNA dimethylallyltransferase, mitochondrial (MOD5)

  • 中文名稱：
    釀酒酵母MOD5重組蛋白
  • 貨號：
    CSB-YP357241SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母MOD5重組蛋白
  • 貨號：
    CSB-EP357241SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母MOD5重組蛋白
  • 貨號：
    CSB-EP357241SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母MOD5重組蛋白
  • 貨號：
    CSB-BP357241SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母MOD5重組蛋白
  • 貨號：
    CSB-MP357241SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MOD5
  • Uniprot No.：
  • 別名：
    MOD5; YOR274W; tRNA dimethylallyltransferase; DMATase; EC 2.5.1.75; Isopentenyl-diphosphate: tRNA isopentenyltransferase; IPP transferase; IPPT; tRNA isopentenyltransferase; IPTase
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-428
  • 氨基酸序列
    MLKGPLKGCL NMSKKVIVIA GTTGVGKSQL SIQLAQKFNG EVINSDSMQV YKDIPIITNK HPLQEREGIP HHVMNHVDWS EEYYSHRFET ECMNAIEDIH RRGKIPIVVG GTHYYLQTLF NKRVDTKSSE RKLTRKQLDI LESTDPDVIY NTLVKCDPDI ATKYHPNDYR RVQRMLEIYY KTGKKPSETF NEQKITLKFD TLFLWLYSKP EPLFQRLDDR VDDMLERGAL QEIKQLYEYY SQNKFTPEQC ENGVWQVIGF KEFLPWLTGK TDDNTVKLED CIERMKTRTR QYAKRQVKWI KKMLIPDIKG DIYLLDATDL SQWDTNASQR AIAISNDFIS NRPIKQERAP KALEELLSKG ETTMKKLDDW THYTCNVCRN ADGKNVVAIG EKYWKIHLGS RRHKSNLKRN TRQADFEKWK INKKETVE
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in the anticodon loop on a specific subset of tRNAs both in the cytosol and the mitochondrion, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A). This modification optimizes the codon:anticodon fit in the ribosome and promotes translational fidelity. Competes with the farnesyl pyrophosphate synthase ERG20 for the common substrate dimethylallyl diphosphate (DMAPP).
  • 基因功能參考文獻：
    1. Mod5 protein binds to tRNA gene complexes and affects local transcriptional silencing. PMID: 23898186
    2. identified Mod5 as a yeast prion protein and found that its prion conversion in yeast regulated the sterol biosynthetic pathway for acquired cellular resistance against antifungal agents; selective pressure by antifungal drugs on yeast facilitated the de novo appearance of Mod5 prion states for cell survival PMID: 22517861
    3. show that Tit1p accommodates a broader range of substrates than Mod5p PMID: 21873461
  • 亞細胞定位：
    Cytoplasm. Mitochondrion. Nucleus. Note=[MOD+] forms multicytoplasmic aggregates that do not colocalize to either mitochondria or nucleus.; [Isoform I]: Cytoplasm. Mitochondrion.; [Isoform II]: Cytoplasm. Nucleus.
  • 蛋白家族：
    IPP transferase family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YOR274W

    STRING: 4932.YOR274W