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  4. Recombinant Salmonella typhimurium Negative regulator of flagellin synthesis (flgM)

Recombinant Salmonella typhimurium Negative regulator of flagellin synthesis (flgM)

  • 中文名稱：
    鼠傷寒沙門菌flgM重組蛋白
  • 貨號：
    CSB-YP326298SXB
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌flgM重組蛋白
  • 貨號：
    CSB-EP326298SXB
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌flgM重組蛋白
  • 貨號：
    CSB-EP326298SXB-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌flgM重組蛋白
  • 貨號：
    CSB-BP326298SXB
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌flgM重組蛋白
  • 貨號：
    CSB-MP326298SXB
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    flgM
  • Uniprot No.：
  • 別名：
    flgM; flgR; mviS; STM1172Negative regulator of flagellin synthesis; Anti-sigma-28 factor
  • 種屬：
    Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-97
  • 氨基酸序列
    MSIDRTSPLK PVSTVQTRET SDTPVQKTRQ EKTSAATSAS VTLSDAQAKL MQPGVSDINM ERVEALKTAI RNGELKMDTG KIADSLIREA QSYLQSK
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Responsible for the coupling of flagellin expression to flagellar assembly by preventing expression of the flagellin genes when a component of the middle class of proteins is defective. It negatively regulates flagellar genes by inhibiting the activity of FliA by directly binding to FliA.
  • 基因功能參考文獻：
    1. Authors demonstrated that (i) FliS and FlgM interact specifically, forming a 1:1 complex, (ii) the FliS binding site on FlgM is proximal to or even overlaps the binding site for FliA, and (iii) FliA competes with FliS for FlgM binding. PMID: 24415724
    2. Based on these results, we conclude that the sigma(28)-FlgM regulatory circuit continuously senses the flagellar hook-basal body assembly process and regulates class 3 gene expression and possibly flagellar numbers in response. PMID: 21166907
    3. defetive gene translation caused by a mutant with single nucleotide substitution in serT PMID: 16352846
    4. secretion into periplasm inhibited by Flk. PMID: 16629666
    5. data demonstrate that sigma(28) is the Type III secretion chaperone for its own anti-sigma factor FlgM. PMID: 16912280

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  • 亞細胞定位：
    Cytoplasm. Note=After completion of the hook-basal body structure, it is exported out of the cell through the completely assembled hook-basal body structures.
  • 蛋白家族：
    FlgM family
  • 數(shù)據(jù)庫鏈接：

    KEGG: stm:STM1172

    STRING: 99287.STM1172