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  4. Recombinant Schizosaccharomyces pombe Telomere length regulator protein rif1 (rif1), partial

Recombinant Schizosaccharomyces pombe Telomere length regulator protein rif1 (rif1), partial

  • 中文名稱：
    龐貝裂殖酵母rif1重組蛋白
  • 貨號：
    CSB-YP839423SXV
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母rif1重組蛋白
  • 貨號：
    CSB-EP839423SXV
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母rif1重組蛋白
  • 貨號：
    CSB-EP839423SXV-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母rif1重組蛋白
  • 貨號：
    CSB-BP839423SXV
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母rif1重組蛋白
  • 貨號：
    CSB-MP839423SXV
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    rif1; SPAC6F6.17; SPAPJ736.01; Telomere length regulator protein rif1
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Negatively regulates telomere length. Appears to play no role in transcriptional silencing of telomeric loci.
  • 基因功能參考文獻：
    1. Genetic screens for identifying cis- and trans-acting factors creating heterochromatin-euchromatin boundaries showed unexpected properties for Rif1 and Taz1. Most cis-acting elements identified came from genomic loci bound by Taz1 and associated with origins of replication repressed by Taz1 and Rif1. Boundary formation by these elements required Taz1 and Rif1 and coincided with altered replication timing. PMID: 28096402
    2. Rif1 is a critical determinant of the origin activation program on the fission yeast chromosomes [Rif1] [strain not found in database] PMID: 22279046
    3. Rif1 localizes to the anaphase mid-region and regulates the resolution of persisting DNA structures. PMID: 27320927
    4. These results suggest that Rif1 recognizes and binds G quadruplex-like structures at selected intergenic regions, thus generating local chromatin structures that may exert long-range suppressive effects on origin firing. PMID: 26436827
    5. Rap1 and Rif1 are each required for a subset of the functions of Taz1 PMID: 16096639

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  • 亞細胞定位：
    Nucleus. Chromosome, telomere. Note=Localized to telomeres. This interaction may be increased by perturbation of telomere structure, for instance by loss of the rap1 protein.
  • 蛋白家族：
    RIF1 family
  • 數(shù)據(jù)庫鏈接：