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  4. Recombinant Xenopus laevis Replication protein A 70 kDa DNA-binding subunit (rpa1), partial

Recombinant Xenopus laevis Replication protein A 70 kDa DNA-binding subunit (rpa1), partial

  • 中文名稱：
    有爪蟾蜍rpa1重組蛋白
  • 貨號(hào)：
    CSB-YP020088XBE
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    有爪蟾蜍rpa1重組蛋白
  • 貨號(hào)：
    CSB-EP020088XBE
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    有爪蟾蜍rpa1重組蛋白
  • 貨號(hào)：
    CSB-EP020088XBE-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    有爪蟾蜍rpa1重組蛋白
  • 貨號(hào)：
    CSB-BP020088XBE
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    有爪蟾蜍rpa1重組蛋白
  • 貨號(hào)：
    CSB-MP020088XBE
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    rpa1; Replication protein A 70 kDa DNA-binding subunit; RP-A p70; Replication factor A protein 1; RF-A protein 1; Single-stranded DNA-binding protein
  • 種屬：
    Xenopus laevis (African clawed frog)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.
  • 基因功能參考文獻(xiàn)：
    1. This study found that the N-terminus of the RPA large subunit interacts with both WRN and DNA2 and is essential for stimulating WRN's 3'->5' helicase activity and DNA2's 5'->3' ss-DNA exonuclease activity. PMID: 26227969
    2. The results provide strong biochemical evidence to link RPA to a specific DSB repair pathway and reveal a novel function of RPA in the generation of 3' ss-DNA for homology-dependent DSB repair. PMID: 21263027
    3. Replication protein A accumulated increasingly on replication-arrested chromatin. PMID: 16798775
    4. RPA1 subunit may directly recognize and bind to the 5-formyluracil on the single-stranded DNA. PMID: 16838837
  • 亞細(xì)胞定位：
    Nucleus. Nucleus, PML body.
  • 蛋白家族：
    Replication factor A protein 1 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: xla:397937

    UniGene: Xl.3957