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  4. Recombinant Xenopus laevis Targeting protein for Xklp2-A (tpx2-a), partial

Recombinant Xenopus laevis Targeting protein for Xklp2-A (tpx2-a), partial

  • 中文名稱：
    有爪蟾蜍tpx2-a重組蛋白
  • 貨號：
    CSB-YP764667XBE
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    有爪蟾蜍tpx2-a重組蛋白
  • 貨號：
    CSB-EP764667XBE
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    有爪蟾蜍tpx2-a重組蛋白
  • 貨號：
    CSB-EP764667XBE-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    有爪蟾蜍tpx2-a重組蛋白
  • 貨號：
    CSB-BP764667XBE
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    有爪蟾蜍tpx2-a重組蛋白
  • 貨號：
    CSB-MP764667XBE
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    tpx2-a
  • Uniprot No.：
  • 別名：
    tpx2-a; Targeting protein for Xklp2-A
  • 種屬：
    Xenopus laevis (African clawed frog)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Spindle assembly factor. Required for normal assembly of mitotic spindles. Mediates the binding kif15 and aurka to spindle microtubules. Required for targeting kif15 to microtubule minus ends. Activates aurka by promoting its autophosphorylation and protects the phosphorylated residue against dephosphorylation.
  • 基因功能參考文獻：
    1. This study identifies TPX2 as the only direct RanGTP target and NEDD1 as the only Aurora A substrate essential for the activation of the RanGTP-dependent microtubule nucleation pathway. PMID: 25532896
    2. TPX2 levels modulate spindle architecture through Eg5 in Xenopus oocyte extract. PMID: 25070954
    3. TPX2 is essential for microtubule nucleation around chromatin PMID: 15385625
    4. Here we identify G205 in Xenopus Aurora A as a key determinant of both intrinsic activity and regulation by TPX2. PMID: 15908779
    5. TPX2 interacts with lamina-associated polypeptide 2 (LAP2). LAP2 localization is disrupted in TPX2-depleted nuclei, suggesting interaction between TPX2 and LAP2 is required for postmitotic nuclear reformation PMID: 16735579
    6. HURP and TPX2 have nonredundant functions essential for chromatin-induced microtubule assembly. PMID: 18799614
    7. Data show that depletion of TPX2 was partially rescued by the addition of excess XMAP215 or EB1, or inhibiting MCAK (a Kinesin-13). PMID: 19369413

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  • 亞細胞定位：
    Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=Localizes during metaphase on spindle microtubules, with a strong enrichment at spindle poles. Localizes to the minus ends of spindle pole and aster microtubules in a dynein- and dynactin-dependent manner.
  • 蛋白家族：
    TPX2 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: xla:398174

    UniGene: Xl.48391