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  4. Recombinant Arabidopsis thaliana High-affinity nitrate transporter 3.1 (NRT3.1)

Recombinant Arabidopsis thaliana High-affinity nitrate transporter 3.1 (NRT3.1)

  • 中文名稱：
    擬南芥NRT3.1重組蛋白
  • 貨號(hào)：
    CSB-CF872221DOA
  • 規(guī)格：
  • 來源：
    in vitro E.coli expression system
  • 其他：

產(chǎn)品詳情

  • 基因名：
    NRT3.1
  • Uniprot No.：
  • 別名：
    NRT3.1; NAR2.1; NAR2.2; WR3; At5g50200; K6A12.6; High-affinity nitrate transporter 3.1; Protein WOUND-RESPONSIVE 3
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長(zhǎng)度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    23-210
  • 氨基酸序列
    AEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLNITWTLSSIGSKREAEFKIIKVK LCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPYDKTLQSTTWTLERDIPTGTYFVRAY AVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHASLDIASICFSVFSVVALVVFFVNEKR KAKIEQSK
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Acts as a dual component transporter with NTR2.1. Required for high-affinity nitrate transport. Acts as a repressor of lateral root initiation. May be involved in targeting NRT2 proteins to the plasma membrane.
  • 基因功能參考文獻(xiàn)：
    1. AtNRT2.5 is predominantly expressed in roots of nitrate-deprived WT plants as a 150 kDa molecular complex with AtNAR2.1 which is the major contributor to constitutive high-affinity nitrate influx in Arabidopsis thaliana. [NAR2.1] PMID: 25474587
    2. Except for AtNRT2.7, all other NRT2 transporters interact strongly with AtNAR2.1. PMID: 22432443
    3. Data demonstrate that an intact two-component complex of AtNRT2.1 and AtNAR2.1 (AtNRT3.1) is localized in the plasma membrane. PMID: 20561257
    4. The constitutive high-affinity influx and nitrate-inducible high-affinity influx (but not the low-affinity influx) of higher plant roots require a functional AtNRT3 gene. [AtNRT3.1] PMID: 16415212
    5. The NRT3.1 gene encodes a key component of the HATS (high-affinity NO3 transport system) and repression of NRT2.1 and NRT3.1 by high nitrates is specifically mediated by the NRT1.1 transporter. [NRT3.1] PMID: 16998085
    6. AtNAR2.1 is essential for high-affinity transport system of nitrate in Arabidopsis.[NAR2.1] PMID: 17012411

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  • 亞細(xì)胞定位：
    Cell membrane; Single-pass membrane protein.
  • 蛋白家族：
    NAR2 family
  • 組織特異性：
    Highly expressed in roots. Detected in shoots.
  • 數(shù)據(jù)庫鏈接：

    KEGG: ath:AT5G50200

    STRING: 3702.AT5G50200.1

    UniGene: At.21028