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  4. Recombinant Drosophila melanogaster Tyramine beta-hydroxylase (Tbh)

Recombinant Drosophila melanogaster Tyramine beta-hydroxylase (Tbh)

  • 中文名稱：
    黑腹果蠅Tbh重組蛋白
  • 貨號(hào)：
    CSB-CF769720DLU
  • 規(guī)格：
  • 來源：
    in vitro E.coli expression system
  • 其他：

產(chǎn)品詳情

  • 基因名：
    Tbh
  • Uniprot No.：
  • 別名：
    Tbh; CG1543; Tyramine beta-hydroxylase
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-670
  • 氨基酸序列
    MLKIPLQLSSQDGIWPARFARRLHHHHQLAYHHHKQEQQQQQQQQQQQQAKQKQKQNGVQ QGRSPTFMPVMLLLLMATLLTRPLSAFSNRLSDTKLHEIYLDDKEIKLSWMVDWYKQEVL FHLQNAFNEQHRWFYLGFSKRGGLADADICFFENQNGFFNAVTDTYTSPDGQWVRRDYQQ DCEVFKMDEFTLAFRRKFDTCDPLDLRLHEGTMYVVWARGETELALEDHQFALPNVTAPH EAGVKMLQLLRADKILIPETELDHMEITLQEAPIPSQETTYWCHVQRLEGNLRRRHHIVQ FEPLIRTPGIVHHMEVFHCEAGEHEEIPLYNGDCEQLPPRAKICSKVMVLWAMGAGTFTY PPEAGLPIGGPGFNPYVRLEVHFNNPEKQSGLVDNSGFRIKMSKTLRQYDAAVMELGLEY TDKMAIPPGQTAFPLSGYCVADCTRAALPATGIIIFGSQLHTHLRGVRVLTRHFRGEQEL REVNRDDYYSNHFQEMRTLHYKPRVLPGDALVTTCYYNTKDDKTAALGGFSISDEMCVNY IHYYPATKLEVCKSSVSEETLENYFIYMKRTEHQHGVHLNGARSSNYRSIEWTQPRIDQL YTMYMQEPLSMQCNRSDGTRFEGRSSWEGVAATPVQIRIPIHRKLCPNYNPLWLKPLEKG DCDLLGECIY
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Converts tyramine into octopamine, a neurotransmitter involved in ovulation and locomotion. Functions in an amine-mediated Bacc-dependent signaling pathway that negatively regulates acute ethanol sensitivity.
  • 基因功能參考文獻(xiàn)：
    1. While octopamine (OA) release reduced body fat, we inferred that tyramine (TA)release has the opposite effect, rescuing the fat deposition phenotype clearly seen in Tbh-deficient Drosophila. PMID: 27759117
    2. Interdomain long-range electron transfer becomes rate-limiting in the Y216A variant of tyramine beta-monooxygenase. PMID: 23320946
    3. Data show that ovulation is defective in females lacking tyramine beta-hydroxylase, resulting in blockage of mature oocytes within the ovaries. PMID: 14623230
    4. Recombinant tyramine beta-monooxygenase requires copper for activity and has a typical type 2 copper electron spin resonance (EPR) spectrum. PMID: 16376104
    5. Octopamine is not the natural signal for flight initiation because flies lacking octopamine [tyramine-beta-hydroxylase null mutants] can fly, though they show markedly different flight-initiation and -maintenance capabilities compared to wild-type flies. PMID: 17928454
    6. the tyramine beta-monooxygenase mechanism is different from that of the mammalian enzyme, dopamine beta-monooxygenase PMID: 18032384

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  • 亞細(xì)胞定位：
    Membrane; Single-pass membrane protein.
  • 蛋白家族：
    Copper type II ascorbate-dependent monooxygenase family
  • 組織特異性：
    Present in head and in neurons innervating the oviduct (at protein level).
  • 數(shù)據(jù)庫鏈接：