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  4. Recombinant Escherichia coli L-alanine exporter AlaE (alaE)

Recombinant Escherichia coli L-alanine exporter AlaE (alaE)

  • 中文名稱：
    Recombinant Escherichia coli L-alanine exporter AlaE(alaE)
  • 貨號：
    CSB-CF354327ENV
  • 規(guī)格：
  • 來源：
    in vitro E.coli expression system
  • 其他：

產(chǎn)品詳情

  • 基因名：
    alaE
  • Uniprot No.：
  • 別名：
    alaE; ygaW; b2670; JW2645; L-alanine exporter AlaE
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-149
  • 氨基酸序列
    MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYSRLVAIPVNILIAWPYG MYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLVVGADWHQIMAAVSSNIVV SMLMGAVYGYFLDYCRRLFKVSRYQQVKA
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Exports L-alanine.
  • 基因功能參考文獻(xiàn)：
    1. the gene expression of the alaE is regulated by Lrp in response to intracellular levels of l-alanine, which eventually leads to intracellular homeostasis of l-alanine concentrations PMID: 28057466
    2. Charged amino acid residues (R45 and D84) in the AlaE transmembrane domain play a pivotal role in L-alanine export. PMID: 27572251
    3. These data suggested that the AlaE-mediated l-alanine extrusion was driven by proton motive force. PMID: 26073055
    4. On the basis of these results, the authors concluded that YgaW is likely to be the physiologically most relevant exporter for L-alanine in Escherichia coli and proposed that the gene be redesignated alaE for alanine export. PMID: 21531828
  • 亞細(xì)胞定位：
    Cell inner membrane; Multi-pass membrane protein.
  • 蛋白家族：
    AlaE exporter family
  • 數(shù)據(jù)庫鏈接：