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  4. Recombinant Escherichia coli Ubiquinol oxidase subunit 2 (cyoA)

Recombinant Escherichia coli Ubiquinol oxidase subunit 2 (cyoA)

  • 中文名稱：
    Recombinant Escherichia coli Ubiquinol oxidase subunit 2(cyoA)
  • 貨號：
    CSB-CF364657ENV
  • 規(guī)格：
  • 來源：
    in vitro E.coli expression system
  • 其他：

產(chǎn)品詳情

  • 基因名：
    cyoA
  • Uniprot No.：
  • 別名：
    cyoA; b0432; JW0422; Cytochrome bo(3 ubiquinol oxidase subunit 2; Cytochrome b562-o complex subunit II; Cytochrome o ubiquinol oxidase subunit 2; Cytochrome o subunit 2; Oxidase bo(3 subunit 2; Ubiquinol oxidase chain B; Ubiquinol oxidase polypeptide II; Ubiquinol oxidase subunit 2
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區(qū)域：
    25-315
  • 氨基酸序列
    CNSALLDPKGQIGLEQRSLILTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSH SNKVEAVVWTVPILIIIFLAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPE QGIATVNEIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYD GISASYSGPGFSGMKFKAIATPDRAAFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVE YFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽：
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Cytochrome bo(3) ubiquinol terminal oxidase is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration. Has proton pump activity across the membrane in addition to electron transfer, pumping 2 protons/electron.
  • 基因功能參考文獻：
    1. Removal of three terminal cytochrome oxidase genes (cydAB, cyoABCD, and cbdAB) and a quinol monooxygenase gene (ygiN) from the Escherichia coli K-12 MG1655 genome resulted in the activation of ArcA aerobically. PMID: 20709841
    2. analysis of translocation of the N-tail and C-tail of the Escherichia coli inner membrane protein CyoA PMID: 16481320
    3. Data show that the distinct domains of CyoA have different translocation requirements (YidC only and Sec/YidC) and confirms that the membrane biogenesis of subunit II of cytochrome oxidase in bacteria and mitochondria have conserved features. PMID: 16488430
    4. CyoA is a substrate of a novel pathway that involves both SecYEG and YidC PMID: 16513637
  • 亞細胞定位：
    Cell inner membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Cytochrome c oxidase subunit 2 family
  • 數(shù)據(jù)庫鏈接：