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  4. Recombinant Human coronavirus NL63 Envelope small membrane protein (E)

Recombinant Human coronavirus NL63 Envelope small membrane protein (E)

  • 貨號(hào)：
    CSB-CF764822HIX
  • 規(guī)格：
    ￥9720
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    E
  • Uniprot No.：
  • 別名：
    E; sM; 4; Envelope small membrane protein; E protein; sM protein
  • 種屬：
    Human coronavirus NL63 (HCoV-NL63)
  • 蛋白長(zhǎng)度：
    Full Length
  • 來源：
    in vitro E.coli expression system
  • 分子量：
    12.0 kDa
  • 表達(dá)區(qū)域：
    1-77aa
  • 氨基酸序列
    MFLRLIDDNGIVLNSILWLLVMIFFFVLAMTFIKLIQLCFTCHYFFSRTLYQPVYKIFLAYQDYMQIAPVPAEVLNV
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    18-23 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    The recombinant HCoV-NL63 E protein protein is a cell-free system in vitro E.coli expressed Full Length protein. In cell-free systems, synthesis of the protein can be carried out in vitro using extracts of whole cells that are compatible with translation. These cell extracts contain all the molecules and enzymes that are needed to transcribe, translate, and post-translationally modify the recombinant protein. With additional supplements of cofactors, E protein proteins can be formed in a few hours. However, this system may not be applicable for the large-scale production of recombinant proteins. Advantages of this system include that proteins can be synthesized without cell culturing; also, it is possible to express many proteins together.

    The HCoV-NL63 E protein is a small, integral, structural membrane protein found in the lipid envelope of mature virus particles. It functions in various stages of the viral life cycle, such as assembly, budding, envelope formation, and pathogenesis. Absence or inactivation of E protein leads to attenuated viruses because of changes in either virion morphology or tropism. The E protein is abundantly expressed inside the infected cells during the replication cycle, but only a small fraction is integrated into the virion membrane. The majority of the E protein is found in intracellular trafficking sites such as the ER, Golgi, and ERGIC, where it aids in HCoV-NL63 assembly and budding. The E protein also plays an important role in viral production and maturation.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Plays a central role in virus morphogenesis and assembly. Acts as a viroporin and self-assembles in host membranes forming pentameric protein-lipid pores that allow ion transport. Also plays a role in the induction of apoptosis.
  • 亞細(xì)胞定位：
    Host Golgi apparatus membrane; Single-pass type III membrane protein.
  • 蛋白家族：
    Alphacoronaviruses E protein family
  • 數(shù)據(jù)庫鏈接：

    KEGG: vg:2943502