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  4. Recombinant Saccharomyces cerevisiae Mitochondrial import inner membrane translocase subunit TIM21 (TIM21)

Recombinant Saccharomyces cerevisiae Mitochondrial import inner membrane translocase subunit TIM21 (TIM21)

  • 中文名稱：
    釀酒酵母TIM21重組蛋白
  • 貨號(hào)：
    CSB-CF344864SVG
  • 規(guī)格：
  • 來(lái)源：
    in vitro E.coli expression system
  • 其他：

產(chǎn)品詳情

  • 基因名：
    TIM21
  • Uniprot No.：
  • 別名：
    TIM21; YGR033C; Mitochondrial import inner membrane translocase subunit TIM21
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長(zhǎng)度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    71-239
  • 氨基酸序列
    ASTFTFSGILVIGAVGISAIVIYLILSELFSPSGDTQLFNRAVSMVEKNKDIRSLLQCDD GITGKERLKAYGELITNDKWTRNRPIVSTKKLDKEGRTHHYMRFHVESKKKIALVHLEAK ESKQNYQPDFINMYVDVPGEKRYYLIKPKLHPVSNSKGFLGIRWGPRKD
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to keep the TOM and the TIM23 complexes in close contact. At some point, it is released from the TOM23 complex to allow protein translocation into the mitochondrial matrix. In the complex, it acts as an antagonist of TIM50 by reducing preprotein accumulation at the TOM23 complex and promotes dissociation of the PAM complex from the TIM23 complex.
  • 基因功能參考文獻(xiàn)：
    1. Three Tim23 motifs bind to a single conserved pocket in Tim21. PMID: 25263020
    2. The signal-driven release of Tim21 from Tim50 promotes recruitment of Pam17 and thus triggers formation of the motor-associated form of the TIM23 complex required for matrix transport. PMID: 23403928
    3. Tim21 binds to the trans site of the TOM complex thus keeping the two translocases in close contact PMID: 15878866
    4. Tim21 interacts with the respiratory chain and promotes membrane-potential-dependent protein sorting into the inner mitochondrial membrane. PMID: 17113393
  • 亞細(xì)胞定位：
    Mitochondrion inner membrane; Single-pass membrane protein.
  • 蛋白家族：
    TIM21 family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: sce:YGR033C

    STRING: 4932.YGR033C