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  4. Recombinant Streptomyces coelicolor pH-gated potassium channel KcsA (kcsA)-VLPs

Recombinant Streptomyces coelicolor pH-gated potassium channel KcsA (kcsA)-VLPs

  • 中文名稱：
    Recombinant Streptomyces coelicolor pH-gated potassium channel KcsA (kcsA)-VLPs
  • 貨號：
    CSB-MP358316FOB
  • 規(guī)格：
  • 其他：

產(chǎn)品詳情

  • 基因名：
    kcsA
  • Uniprot No.：
  • 種屬：
    Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
  • 蛋白長度：
    Full Length
  • 來源：
    Mammalian cell
  • 表達(dá)區(qū)域：
    1-160aa
  • 氨基酸序列
    MPPMLSGLLARLVKLLLGRHGSALHWRAAGAATVLLVIVLLAGSYLAVLAERGAPGAQLITYPRALWWSVETATTVGYGDLYPVTLWGRLVAVVVMVAGITSFGLVTAALATWFVGREQERRGHFVRHSEKAAEEAYTRTTRALHERFDRLERMLDDNRR
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    C-terminal 10xHis-tagged
    If you have specified tag type, please tell us and we will check if it's possible to develop.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from PBS, 6% Trehalose, pH 7.4.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項(xiàng)：
    The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex. Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel.
  • 基因功能參考文獻(xiàn)：
    1. Studied the expression, purification, liposome reconstitution and functional validation of uniformly (13)C and (15)N isotope labeled KcsA, a bacterial potassium channel that has high homology with mammalian channels, for solid-state NMR studies. PMID: 23916531
    2. Results reveal that a decrease in pH introduces major conformational rearrangements associated with channel opening in the KcsA cytoplasmic domain. PMID: 19959477
    3. analysis of phospholipid interactions with the potassium channel KcsA PMID: 16272446
    4. analysis of the potassium channel KcsA inactivation by the Shaker B "ball" peptide PMID: 18430729
  • 亞細(xì)胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Potassium channel family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sco:SCO7660

    STRING: 100226.SCO7660