ACLY
ACLY,即ATP檸檬酸裂合酶,別名包括ATP-citratelyase、ACL、ATPCL、CLATP等。ACLY由人類ACLY基因編碼,該基因位于17號染色體的q21.2區(qū)域。ACLY是一種關鍵的代謝酶,催化ATP依賴的檸檬酸和輔酶A(CoA)轉化為草酰乙酸和乙酰輔酶A(Acetyl-CoA)的反應。乙酰輔酶A是脂肪酸代謝、膽固醇生物合成以及蛋白質乙?;彤愇於┗揎椀年P鍵前體。在生物學意義上,ACLY通過連接糖代謝和脂肪酸合成,對細胞的代謝調控和能量平衡具有重要作用。ACLY在多種類型的癌癥中表達上調,其抑制能夠誘導癌細胞增殖停滯,因此被認為是癌癥治療的潛在靶點。
熱銷產(chǎn)品
Recombinant Human ATP-citRate synthase (ACLY), partial (CSB-EP001158HU)
驗證數(shù)據(jù)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
ACLY Recombinant Monoclonal Antibody (CSB-RA712206A0HU)
驗證數(shù)據(jù)

Western Blot
Positive WB detected in: L02 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, A549 whole cell lysate
All lanes: ACLY antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 121, 120, 92 kDa
Observed band size: 120 kDa

Immunofluorescence staining of HepG2 Cells with CSB-RA712206A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Overlay histogram showing Hela cells stained with CSB-RA712206A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1μg/1*106 cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1μg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
ACLY Antibodies
ACLY for Homo sapiens (Human)
產(chǎn)品貨號 | 產(chǎn)品名稱 | 種屬反應性 | 應用類型 |
---|---|---|---|
CSB-PA001158GA01HU | ACLY Antibody | Human,Mouse,Rat | ELISA,WB,IF |
CSB-PA000957 | ACLY Antibody | Human,Mouse,Rat,Monkey | WB, IF, ELISA |
CSB-PA020036 | Phospho-ACLY (S455) Antibody | Human,Mouse,Rat,Monkey | WB, IHC, ELISA |
CSB-PA056430 | ACLY Antibody | Human,Mouse,Rat | ELISA,IHC |
CSB-PA189814 | ACLY Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA001158LA01HU | ACLY Antibody | Human | ELISA, WB, IHC, IF |
CSB-RA712206A0HU | ACLY Recombinant Monoclonal Antibody | Human | ELISA, WB, IF, FC |
ACLY Proteins
ACLY Proteins for Homo sapiens (Human)
產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
---|---|---|
CSB-YP001158HU CSB-BP001158HU CSB-MP001158HU CSB-EP001158HU-B |
Recombinant Human ATP-citrate synthase (ACLY), partial | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP001158HU | Recombinant Human ATP-citRate synthase (ACLY), partial | E.coli |