BRD4
BRD4,即溴結(jié)構(gòu)域蛋白4,別名BRD4。BRD4是一種屬于溴結(jié)構(gòu)域和超末端結(jié)構(gòu)域(BET)家族的蛋白,主要通過識別乙?;M蛋白來調(diào)節(jié)基因表達。BRD4的作用機制主要涉及其與轉(zhuǎn)錄因子的相互作用。BRD4通過其兩個溴結(jié)構(gòu)域(BD1和BD2)結(jié)合乙?;慕M蛋白,定位于目標(biāo)基因的啟動子或增強子區(qū)域,促進相關(guān)基因的轉(zhuǎn)錄。此外,BRD4還通過招募轉(zhuǎn)錄延伸因子和其他調(diào)節(jié)因子,增強RNA聚合酶II的活性,從而促進基因的轉(zhuǎn)錄伸長。BRD4在細胞周期、增殖和凋亡等生理過程中起著關(guān)鍵作用。研究表明,BRD4的異常表達與多種惡性腫瘤的發(fā)生發(fā)展相關(guān),包括急性髓系白血病、多發(fā)性骨髓瘤、乳腺癌和膠質(zhì)母細胞瘤等。BRD4不僅參與腫瘤細胞的增殖和轉(zhuǎn)移,還在DNA損傷修復(fù)和端粒維持中發(fā)揮重要作用。因此,BRD4成為了癌癥治療的一個重要靶點。
熱銷產(chǎn)品
Recombinant Human Bromodomain-containing protein 4 (BRD4), partial (CSB-EP002802HU)
驗證數(shù)據(jù)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
BRD4 Recombinant Monoclonal Antibody (CSB-RA152430A0HU)
驗證數(shù)據(jù)

Western Blot
Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
All lanes: BRD4 antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 153, 81, 89 kDa
Observed band size: 153 kDa

IHC image of CSB-RA152430A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA152430A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
BRD4 Antibodies
BRD4 for Homo sapiens (Human)
產(chǎn)品貨號 | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
---|---|---|---|
CSB-PA577233 | BRD4 Antibody | Human,Mouse | ELISA,WB |
CSB-PA249846 | BRD4 Antibody | Human,Mouse | ELISA,WB |
CSB-PA002802LA01HU | BRD4 Antibody | Human | ELISA, IF |
CSB-PA002802LD01HU | BRD4 Antibody, Biotin conjugated | Human | ELISA |
CSB-RA152430A0HU | BRD4 Recombinant Monoclonal Antibody | Human | ELISA, WB, IHC |
BRD4 Proteins
BRD4 Proteins for Homo sapiens (Human)
產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
---|---|---|
CSB-YP002802HU CSB-BP002802HU CSB-MP002802HU CSB-EP002802HU-B |
Recombinant Human Bromodomain-containing protein 4 (BRD4), partial | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP002802HU | Recombinant Human Bromodomain-containing protein 4 (BRD4), partial | E.coli |
CSB-EP002802HU3 | Recombinant Human Bromodomain-containing protein 4 (BRD4), partial | E.coli |
CSB-EP002802HU2 | Recombinant Human Bromodomain-containing protein 4 (BRD4), partial | E.coli |
CSB-YP002802HU3 | Recombinant Human Bromodomain-containing protein 4 (BRD4), partial | Yeast |
CSB-YP002802HU3b0 | Recombinant Human Bromodomain-containing protein 4 (BRD4), partial | Yeast |
BRD4 Proteins for Mus musculus (Mouse)
產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
---|---|---|
CSB-YP887677MO CSB-EP887677MO CSB-BP887677MO CSB-MP887677MO CSB-EP887677MO-B |
Recombinant Mouse Bromodomain-containing protein 4 (Brd4), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
BRD4 ELISA Kit
BRD4 ELISA Kit for Homo sapiens (Human)
產(chǎn)品貨號 | 產(chǎn)品名稱 | 樣本類型 | 靈敏度 |
---|---|---|---|
CSB-EL002802HU | Human Bromodomain-containing protein 4(BRD4) ELISA kit | serum, plasma, tissue homogenates | 4.68 pg/mL |