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GAPDH

GAPDH,即甘油醛-3-磷酸脫氫酶,別名G3PDH。GAPDH是一種多功能酶,主要參與糖酵解過程中的關(guān)鍵步驟,即催化甘油醛-3-磷酸轉(zhuǎn)化為1,3-二磷酸甘油酸,并在此過程中釋放能量(ATP)。GAPDH在生物界中非常保守,單體分子量在30kD-40kD之間,天然狀態(tài)的GAPDH主要以四聚體形式存在。除了在能量代謝中的作用,GAPDH還具有多種其他功能,包括催化微管聚合、參與蛋白磷酸化修飾、膜融合和膜轉(zhuǎn)運、促進細胞凋亡、調(diào)節(jié)蛋白質(zhì)的表達、參與DNA損傷修復(fù)、作為轉(zhuǎn)鐵蛋白受體等。GAPDH不僅在糖代謝中發(fā)揮關(guān)鍵作用,其異常表達與多種疾病相關(guān),包括神經(jīng)退行性疾病、糖尿病、癌癥等,且在細胞增殖和腫瘤發(fā)生中可能扮演重要角色。

熱銷產(chǎn)品

Recombinant Human Epidermal growth factor receptor (EGFR), partial (Active) (CSB-MP007479HU)

驗證數(shù)據(jù)

CSB-MP007479HU

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

CSB-MP007479HU

Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP007479HU could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) EGFR.

CSB-MP007479HU

Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP007479HU could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) EGFR.

CSB-MP007479HU

Measured by its binding ability in a functional ELISA. Immobilized EGFR at 1 μg/ml can bind Anti-EGFR recombinant antibody, the EC50 of human EGFR protein is 2.867-3.571 ng/ml.

CSB-MP007479HU

Human EGF protein captured on COOH chip can bind Human EGFR protein, his and Myc tag (CSB-MP007479HU) with an affinity constant of 11.9nM as detected by LSPR Assay.

CSB-MP007479HU

Activity
Measured by its binding ability in a functional ELISA. Immobilized EGFR at 1 μg/ml can bind Cetuximab, the EC50 of human EGFR protein is 0.6919-1.047 ng/ml.

CSB-MP007479HU

The purity of EGFR was greater than 95% as determined by SEC-HPLC

EGFR Recombinant Monoclonal Antibody (CSB-RA159341MA1HU)

驗證數(shù)據(jù)

CSB-RA159341MA1HU

IHC image of CSB-RA159341MA1HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

CSB-RA159341MA1HU

IHC image of CSB-RA159341MA1HU diluted at 1:50 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

CSB-RA159341MA1HU

Overlay Peak curve showing NIH/3T3 cells stained with CSB-RA159341MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

GAPDH Antibodies

GAPDH for Homo sapiens (Human)

GAPDH for Chicken

GAPDH for H, R, M, Mk, Dg, C, Hm, Rb, Pg, Sh, Insect, Yeast

GAPDH for H, R, M, Mk, Dg, C, Hm, Rb, Pg, Sh

GAPDH for Cricetulus griseus

GAPDH Proteins

GAPDH Proteins for Cavia porcellus (Guinea pig)

GAPDH Proteins for Bos taurus (Bovine)

GAPDH Proteins for Mus musculus (Mouse)

GAPDH Proteins for Oryctolagus cuniculus (Rabbit)

GAPDH Proteins for Gallus gallus (Chicken)

GAPDH Proteins for Sus scrofa (Pig)

GAPDH Proteins for Homo sapiens (Human)

GAPDH Proteins for Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)

GAPDH Proteins for Columba livia (Domestic pigeon)

GAPDH Proteins for Meleagris gallopavo (Common turkey)

GAPDH Proteins for Mustela putorius furo (European domestic ferret) (Mustela furo)

GAPDH Proteins for Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)

GAPDH Proteins for Xenopus laevis (African clawed frog)

GAPDH Proteins for Mesocricetus auratus (Golden hamster)

GAPDH Proteins for Rattus norvegicus (Rat)

GAPDH Proteins for Pelodiscus sinensis (Chinese softshell turtle) (Trionyx sinensis)

GAPDH Proteins for Pongo abelii (Sumatran orangutan)

GAPDH Proteins for Danio rerio (Zebrafish) (Brachydanio rerio)

GAPDH Proteins for Felis catus (Cat) (Felis silvestris catus)

GAPDH Proteins for Ovis aries (Sheep)

GAPDH Proteins for Canis familiaris (Dog) (Canis lupus familiaris)

GAPDH Proteins for Glossina morsitans morsitans (Savannah tsetse fly)

GAPDH ELISA Kit

GAPDH ELISA Kit for Homo sapiens (Human)