IL-23（白細(xì)胞介素-23）是一種由IL-23p19和IL-12/IL-23p40兩個(gè)亞基組成的異源二聚體細(xì)胞因子，與IL-12共享p40亞基。IL-23主要通過(guò)與其受體IL-23受體相互作用來(lái)激活下游信號(hào)通路，發(fā)揮生物學(xué)功能。IL-23受體由IL-12受體β1（IL-12Rβ1）和IL-23受體兩個(gè)亞基組成，是一種跨膜蛋白。IL-23在機(jī)體的炎性免疫反應(yīng)和IL-17的產(chǎn)生過(guò)程中發(fā)揮重要作用，主要作用于Th17細(xì)胞，促進(jìn)其增殖與穩(wěn)定，并誘導(dǎo)Th17細(xì)胞產(chǎn)生IL-17A、IL-17F、IL-22等細(xì)胞因子，參與多種疾病的發(fā)生發(fā)展。 IL-23參與的信號(hào)通路主要包括JAK/STAT、MAPK和PI3K等信號(hào)轉(zhuǎn)導(dǎo)途徑。當(dāng)IL-23與其受體結(jié)合后，觸發(fā)IL-12Rβ1和IL-23R的異源二聚化，激活這些信號(hào)通路。IL-23通過(guò)RORγt和STAT3等轉(zhuǎn)錄因子發(fā)揮作用，促進(jìn)Th17細(xì)胞的分化和效應(yīng)子功能。此外，IL-23還與腫瘤免疫、自身免疫性疾病的發(fā)生發(fā)展有關(guān)，例如通過(guò)抑制細(xì)胞毒性T細(xì)胞在腫瘤組織中的浸潤(rùn)促進(jìn)腫瘤生長(zhǎng)，IL-23R的遺傳變異體與多種腫瘤的發(fā)生有關(guān)，血清IL-23水平的升高與某些癌癥的發(fā)展階段相關(guān)。因此，IL-23在調(diào)節(jié)免疫反應(yīng)、促進(jìn)炎癥反應(yīng)以及參與腫瘤免疫和自身免疫性疾病的發(fā)生發(fā)展中發(fā)揮著關(guān)鍵作用。

熱銷產(chǎn)品

IL12&IL23 monoclonal antibody (CSB-RA011587MA1HU)

驗(yàn)證數(shù)據(jù)

The Binding Activity of IL12B&IL12A with Anti-IL12/IL23 monoclonal antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human IL12B&IL12A (CSB-MP4155HU) at 1 μg/mL can bind Anti-IL12/IL23 recombinant antibody, the EC₅₀ is 1.042-1.545 ng/mL.

IHC image of CSB-RA011587MA1HU diluted at 1:50 and staining in paraffin-embedded human lymphnode tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.