NPHS1
NPHS1,別稱CNF、NPHN,是腎小球?yàn)V過屏障結(jié)構(gòu)中的關(guān)鍵蛋白,位于足細(xì)胞裂孔隔膜。該蛋白在維持腎小球?yàn)V過功能中起著至關(guān)重要的作用,其缺陷與芬蘭型先天性腎病綜合癥相關(guān),導(dǎo)致蛋白質(zhì)大量泄漏至尿液中,引發(fā)蛋白尿。NPHS1蛋白的表達(dá)和功能異常,不僅影響腎小球的正常過濾功能,還可能對(duì)心血管系統(tǒng)的發(fā)展造成影響。目前,針對(duì)NPHS1相關(guān)疾病的藥物研發(fā)主要集中在抗-Nephrin抗體,這類抗體在動(dòng)物模型中顯示出誘導(dǎo)蛋白尿和腎病綜合征的潛力。然而,在人體內(nèi)的應(yīng)用仍需謹(jǐn)慎評(píng)估,因?yàn)榭?Nephrin抗體可能成為腎移植后復(fù)發(fā)的發(fā)病機(jī)制之一。此外,對(duì)Nephrin蛋白不同表位和抗體的相互作用機(jī)制研究,以及開發(fā)針對(duì)抗-Nephrin抗體陽性患者的特異性藥物,是未來研究的重點(diǎn)方向。
熱銷產(chǎn)品
Recombinant Human Nephrin (NPHS1), partial (CSB-EP015988HU)
驗(yàn)證數(shù)據(jù)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
NPHS1 Antibody (CSB-PA015988LA01HU)
驗(yàn)證數(shù)據(jù)

WPositive WB detected in: JK whole cell lysate30μg), U251 whole cell lysate(30μg), MCF7 whole cell lysate(30μg), 293T whole cell lysate(30μg)
All lanes: NPHS1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 135,131 kDa
Observed band size: 135 kDa
Exposure time:120s

IHC image of CSB-PA015988LA01HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA015988LA01HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
Human Nephrin ELISA Kit (CSB-E09886h)
驗(yàn)證數(shù)據(jù)

貨號(hào):CSB-E09886h
規(guī)格:96T/48T
靈敏度:0.39 ng/ml
檢測(cè)范圍:1.56 ng/ml-100ng/ml
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
NPHS1 Antibodies
NPHS1 for Homo sapiens (Human)
產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
---|---|---|---|
CSB-PA015988LA01HU | NPHS1 Antibody | Human | ELISA, WB, IHC |
CSB-PA015988LB01HU | NPHS1 Antibody, HRP conjugated | Human | ELISA |
CSB-PA015988LD01HU | NPHS1 Antibody, Biotin conjugated | Human | ELISA |
CSB-PA020148 | NPHS1 Antibody | Human,Mouse,Rat | IHC, ELISA |
CSB-PA909441 | NPHS1 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA967591 | NPHS1 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
NPHS1 Proteins
NPHS1 Proteins for Homo sapiens (Human)
產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
---|---|---|
CSB-EP015988HU | Recombinant Human Nephrin (NPHS1), partial | E.coli |
NPHS1 Proteins for Homo sapiens (Human)
產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
---|---|---|
CSB-MP015988HU | Recombinant Human Nephrin (NPHS1), partial | Mammalian cell |
NPHS1 ELISA Kit
NPHS1 ELISA Kit for Homo sapiens (Human)
產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 樣本類型 | 靈敏度 |
---|---|---|---|
CSB-E09886h | Human Nephrin ELISA Kit | serum, plasma, tissue homogenates | 0.39 ng/ml |