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NSUN2

NSUN2,即NOP2/Sun域家族成員2,是一種關(guān)鍵的tRNA甲基轉(zhuǎn)移酶,別名MISU、SAKI等。它源自人類(lèi)基因表達(dá),負(fù)責(zé)催化RNA分子中的m5C甲基化修飾,這一過(guò)程對(duì)RNA穩(wěn)定性、翻譯效率及蛋白質(zhì)功能至關(guān)重要。NSUN2在細(xì)胞增殖、發(fā)育及分化中扮演核心角色,其異常表達(dá)與多種癌癥的發(fā)生、進(jìn)展及不良預(yù)后緊密相關(guān)。因此,NSUN2不僅是生物學(xué)研究的重要對(duì)象,也是癌癥治療領(lǐng)域潛在的靶標(biāo)。通過(guò)調(diào)控NSUN2的表達(dá)或功能,可為癌癥的診斷、治療及預(yù)后評(píng)估提供新的策略。

熱銷(xiāo)產(chǎn)品

Recombinant Human RNA cytosine C(5)-methyltransferase NSUN2 (NSUN2) (CSB-EP626626HU)

驗(yàn)證數(shù)據(jù)

CSB-EP626626HU

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

NSUN2 Antibody (CSB-PA626626LA01HU)

驗(yàn)證數(shù)據(jù)

CSB-PA626626LA01HU

Western Blot
Positive WB detected in: HepG2 whole cell lysate, Rat liver tissue, Mouse spleen tissue
All lanes: NSUN2 antibody at 6.7μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 87, 83, 60 kDa
Observed band size: 87 kDa

CSB-PA626626LA01HU

Immunofluorescence staining of Hela cells with CSB-PA626626LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CSB-PA626626LA01HU

IHC image of CSB-PA626626LA01HU diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

NSUN2 Antibodies

NSUN2 for Homo sapiens (Human)

NSUN2 Proteins

NSUN2 Proteins for Homo sapiens (Human)