P4HA2，即脯氨酰4-羥化酶亞基2，別名包括 2-oxoglutarate-4-dioxygenase subunit alpha-2、4 PH alpha 2、4-PH alpha-2、C-P4Halpha(II)。它是一種由P4HA2基因編碼的蛋白質(zhì)，屬于脯氨酰4-羥化酶的α亞基，是膠原蛋白合成的關(guān)鍵酶之一。P4HA2的作用機(jī)制主要是催化膠原蛋白前體鏈中脯氨酸殘基的4-羥化，這一過程對(duì)于膠原蛋白的正確三螺旋結(jié)構(gòu)形成至關(guān)重要。P4HA2參與了細(xì)胞外基質(zhì)的重塑，影響腫瘤細(xì)胞的極性、運(yùn)動(dòng)和信號(hào)轉(zhuǎn)導(dǎo)。P4HA2的表達(dá)與多種實(shí)體腫瘤的侵襲、轉(zhuǎn)移密切相關(guān)，并且可以調(diào)控腫瘤相關(guān)基因表達(dá)、增強(qiáng)腫瘤細(xì)胞惡性表型表達(dá)及耐藥性形成。在腫瘤微環(huán)境中，P4HA2通過促進(jìn)膠原蛋白沉積與硬化重塑腫瘤微環(huán)境，進(jìn)而促進(jìn)腫瘤進(jìn)展。此外，P4HA2還通過調(diào)節(jié)細(xì)胞代謝、促進(jìn)腫瘤起始細(xì)胞存活、參與血管基底膜生成及調(diào)控腫瘤相關(guān)基因表達(dá)等多種作用機(jī)制促進(jìn)腫瘤細(xì)胞增殖、黏附和轉(zhuǎn)移。

熱銷產(chǎn)品

P4HA2 Antibody (CSB-PA017340ESR2HU)

驗(yàn)證數(shù)據(jù)

Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA017340ESR2HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA017340ESR2HU at dilution of 1:100

Immunoprecipitating P4HA2 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of (1μg) instead of CSB-PA017340ESR2HU in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA017340ESR2HU (4μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)