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POLR2A

POLR2A編碼RNA聚合酶II的最大亞基Rpb1,該蛋白也被稱(chēng)作POLR2或RPB1,是合成信使RNA的核心催化單元。作為轉(zhuǎn)錄過(guò)程的核心引擎,POLR2A通過(guò)與DNA模板結(jié)合,將遺傳信息轉(zhuǎn)化為前體mRNA,其羧基末端結(jié)構(gòu)域的動(dòng)態(tài)磷酸化狀態(tài)調(diào)控著轉(zhuǎn)錄起始與延伸的轉(zhuǎn)換。POLR2A功能異常與多種惡性腫瘤密切相關(guān),尤其在膠質(zhì)母細(xì)胞瘤、結(jié)直腸癌中常出現(xiàn)基因擴(kuò)增現(xiàn)象,導(dǎo)致細(xì)胞周期失控和基因組不穩(wěn)定性加劇。POLR2A的合成致死效應(yīng)成為抗癌藥物開(kāi)發(fā)新思路,其抑制劑α-鵝膏蕈堿的衍生化合物已進(jìn)入臨床試驗(yàn),通過(guò)特異性阻斷腫瘤細(xì)胞轉(zhuǎn)錄過(guò)程誘導(dǎo)凋亡。2021年Science期刊報(bào)道的抗體偶聯(lián)藥物STT-568,正是利用POLR2A靶向策略在實(shí)體瘤治療中展現(xiàn)潛力。

熱銷(xiāo)產(chǎn)品

Phospho-POLR2A (S2) Recombinant Monoclonal Antibody (CSB-RA018327A02phHU)

驗(yàn)證數(shù)據(jù)

CSB-RA018327A02phHU

Western Blot
Positive WB detected in Hela whole cell lysate,A549 whole cell lysate,293 whole cell lysate
All lanes Phospho-POLR2A antibody at 1.02μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 270 KDa
Observed band size: 270 KDa

CSB-RA018327A02phHU

IHC image of CSB-RA018327A02phHU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

CSB-RA018327A02phHU

Immunofluorescence staining of Hela cells with CSB-RA018327A02phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

CSB-RA018327A02phHU

Immunoprecipitating Phospho-POLR2A in Hela whole cell lysate
Lane 1: Rabbit control IgG(1μg)instead of CSB-RA018327A02phHU in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA018327A02phHU(3μg)+ Hela whole cell lysate(1mg)
Lane 3: Hela whole cell lysate (20μg)

POLR2A Antibodies

POLR2A for Homo sapiens (Human)

POLR2A Proteins

POLR2A Proteins for Homo sapiens (Human)