ROBO1
ROBO1全稱為Roundabout同源受體1,也被稱為DUTT2或SAX3,屬于軸突導(dǎo)向受體家族。ROBO1蛋白通過結(jié)合Slit配體調(diào)控神經(jīng)細(xì)胞遷移和軸突導(dǎo)向,在胚胎發(fā)育中參與神經(jīng)網(wǎng)絡(luò)構(gòu)建,在腫瘤血管生成和癌細(xì)胞轉(zhuǎn)移中同樣發(fā)揮重要作用。ROBO1/Slit信號(hào)異常與肺癌、乳腺癌和結(jié)直腸癌等惡性腫瘤的侵襲性增強(qiáng)相關(guān),其過表達(dá)可能通過激活MAPK和PI3K-AKT通路促進(jìn)腫瘤細(xì)胞存活與遷移。ROBO1基因缺失或突變還與先天性心臟病、智力發(fā)育障礙存在關(guān)聯(lián)。目前針對(duì)ROBO1的藥物開發(fā)聚焦于抗腫瘤領(lǐng)域,已有研究團(tuán)隊(duì)開發(fā)出特異性單克隆抗體,在動(dòng)物模型中顯示出抑制腫瘤轉(zhuǎn)移的效果。ROBO1的雙重作用機(jī)制使其在神經(jīng)修復(fù)和抗腫瘤領(lǐng)域都具有潛在開發(fā)價(jià)值。
熱銷產(chǎn)品
ROBO1 Recombinant Monoclonal Antibody (CSB-RA896760MA1HU)
驗(yàn)證數(shù)據(jù)

IHC image of CSB-RA896760MA1HU diluted at 1:30 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA896760MA1HU diluted at 1:30 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of A549 cell with CSB-RA896760MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescence staining of Hela cell with CSB-RA896760MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Overlay Peak curve showing Hela cells surface stained with CSB-RA896760MA1HU (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,025 events was performed.
Human Roundabout homolog 1(ROBO1) ELISA kit (CSB-EL020054HU)
驗(yàn)證數(shù)據(jù)

貨號(hào):CSB-EL020054HU
規(guī)格:96T/48T
靈敏度:0.039 ng/mL
檢測范圍:0.156 ng/mL-10 ng/mL
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ROBO1 Antibodies
ROBO1 for Homo sapiens (Human)
產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
---|---|---|---|
CSB-PA020054GA01HU | ROBO1 Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA896760LA01HU | ROBO1 Antibody | Human | ELISA, WB, IF |
CSB-PA896760LB01HU | ROBO1 Antibody, HRP conjugated | Human | ELISA |
CSB-PA896760LC01HU | ROBO1 Antibody, FITC conjugated | Human | |
CSB-PA896760LD01HU | ROBO1 Antibody, Biotin conjugated | Human | ELISA |
CSB-RA896760MA1HU | ROBO1 Recombinant Monoclonal Antibody | Human | ELISA, IHC, IF, FC |
ROBO1 Proteins
ROBO1 Proteins for Homo sapiens (Human)
產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
---|---|---|
CSB-YP896760HU CSB-EP896760HU CSB-BP896760HU CSB-EP896760HU-B |
Recombinant Human Roundabout homolog 1 (ROBO1), partial | Yeast E.coli Baculovirus In Vivo Biotinylation in E.coli |
ROBO1 ELISA Kit
ROBO1 ELISA Kit for Homo sapiens (Human)
產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 樣本類型 | 靈敏度 |
---|---|---|---|
CSB-EL020054HU | Human Roundabout homolog 1(ROBO1) ELISA kit | serum, plasma, tissue homogenates | 0.039 ng/mL |