TSPAN8，別名CO-029/TM4SF3。它是一種四跨膜蛋白超家族(TM4SF)的成員，主要在多種腫瘤中高表達(dá)，并可能通過(guò)促進(jìn)血管再生以及增強(qiáng)腫瘤細(xì)胞遷移和侵襲等促進(jìn)腫瘤的轉(zhuǎn)移與進(jìn)展。TSPAN8的作用機(jī)制與其在細(xì)胞膜上的定位有關(guān)，它可以通過(guò)形成同源或異源二聚體與其他蛋白質(zhì)分子在質(zhì)膜上形成多聚體復(fù)合物，從而發(fā)揮功能。此外，TSPAN8還可以通過(guò)與非整合素伴侶如EWI-F、EPCAM、CD13、PKC和PI4KII等相互結(jié)合。TSPAN8在生物學(xué)上的意義主要體現(xiàn)在其對(duì)腫瘤發(fā)生發(fā)展中的調(diào)控作用。研究發(fā)現(xiàn)，TSPAN8在乳腺癌進(jìn)展中EGF/EGFR信號(hào)通路被激活，通過(guò)SOX9上調(diào)TSPAN8轉(zhuǎn)錄。TSPAN8的高表達(dá)與腫瘤干細(xì)胞的特性相關(guān)，并且與治療抵抗和不良預(yù)后有關(guān)。TSPAN8還可以通過(guò)激活PI3K-AKT信號(hào)通路增強(qiáng)癌細(xì)胞的抗凋亡能力。此外，TSPAN8的表達(dá)還與腫瘤微環(huán)境中的細(xì)胞間通訊有關(guān)，它在外泌體中富集，可能通過(guò)細(xì)胞外囊泡在細(xì)胞間進(jìn)行信號(hào)傳遞，影響腫瘤轉(zhuǎn)移。因此，TSPAN8是腫瘤治療中的一個(gè)潛在靶點(diǎn)。

熱銷(xiāo)產(chǎn)品

TSPAN8 Recombinant Monoclonal Antibody (CSB-RA025166MA1HU)

驗(yàn)證數(shù)據(jù)

The Binding Activity of TSPAN8 with Anti-TSPAN8 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human TSPAN8 (CSB-MP025166HU) at 5 μg/mL can bind Anti-TSPAN8 recombinant antibody. The EC₅₀ is 2.261-2.623 ng/mL.The VLPs (CSB-MP3838) is negative control.

Untransfected MC38 cells (green line) and transfected Human TSPAN8 MC38 stable cells (red line) were stained with anti-TSPAN8 antibody (CSB-RA025166MA1HU) (2μg/1*10⁶cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

The purity of TSPAN8 was greater than 95% as determined by SEC-HPLC