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  4. Bovine Alpha-2-HS-glycoprotein(AHSG) ELISA kit

Bovine Alpha-2-HS-glycoprotein(AHSG) ELISA kit

  • 中文名稱：
    牛α2-HS糖蛋白(AHSG)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL001485BO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛α2-HS糖蛋白(AHSG)酶聯(lián)免疫試劑盒（CSB-EL001485BO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AHSG含量。AHSG（α2-HS糖蛋白）是一種由肝臟分泌的血漿糖蛋白，與多種疾病如糖尿病、心血管疾病和結(jié)石等密切相關(guān)。其具有高度的遺傳多態(tài)性，其基因多態(tài)性與糖尿病、血管鈣化等疾病的易感性有關(guān)。AHSG在細(xì)胞過程中發(fā)揮多種作用，如促進(jìn)內(nèi)吞作用、調(diào)節(jié)骨的礦物質(zhì)相、影響骨穩(wěn)態(tài)等。試劑盒檢測范圍為125 ng/mL-8000 ng/mL，本產(chǎn)品可為畜牧業(yè)中牛群健康研究、營養(yǎng)代謝調(diào)控實驗提供數(shù)據(jù)支持，例如骨骼發(fā)育研究、炎癥相關(guān)疾病模型構(gòu)建或免疫系統(tǒng)功能探索等科研方向，使用者可通過檢測血清/血漿樣本評估生理狀態(tài)，或分析肝、腎等組織勻漿中的AHSG表達(dá)水平。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    AHSG ELISA Kit; FETUAAlpha-2-HS-glycoprotein ELISA Kit; Asialofetuin ELISA Kit; Fetuin-A ELISA Kit
  • 縮寫：
    AHSG
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    125 ng/mL-8000 ng/mL
  • 靈敏度：
    31.25 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    Quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine AHSG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 96  
    Range % 92-98  
    1:200 Average % 97  
    Range % 93-102  
    1:400 Average % 102  
    Range % 98-106  
    1:800 Average % 88  
    Range % 82-96  
  • 回收率：
    The recovery of bovine AHSG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-99  
    EDTA plasma (n=4) 104 99-108  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    8000 0.073 0.072 0.073    
    4000 0.138 0.136 0.137    
    2000 0.255 0.242 0.249    
    1000 0.435 0.447 0.441    
    500 0.675 0.686 0.681    
    250 1.038 1.049 1.044    
    125 1.347 1.248 1.298    
    0 1.593 1.695 1.644    
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Suggested to have lymphocyte stimulating properties, lipid binding capability and to bind thyroid hormone.
  • 基因功能參考文獻(xiàn)：
    1. two glycoproteins, AHSG and HPX, represent a pivotal glycoprotein of the cytoprotective activity for WN1316, showing a concrete evidence for the novel glycan-independent function of serum glycoproteins in neuroprotective drug efficacy. PMID: 29016670
    2. Site-specific analysis of the O-glycosylation of bovine fetuin by electron-transfer dissociation mass spectrometry. PMID: 24907489
    3. These experiments revealed a second mechanism of long-term mineral ion stabilization by the fetuin-A that is complementary to the formation of calciprotein particles. PMID: 21156141
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Fetuin family
  • 組織特異性：
    Liver and bone.
  • 數(shù)據(jù)庫鏈接：