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  4. Human Interleukin 12,IL-12/P70 ELISA Kit

Human Interleukin 12,IL-12/P70 ELISA Kit

  • 中文名稱：
    人白介素12(IL-12/P70)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E04599h-IS
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人白介素12(IL-12/P70)酶聯(lián)免疫試劑盒（CSB-E04599h-IS）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液樣本中的IL-12/P70含量。IL-12/P70是重要的細胞因子靶點。它在免疫調節(jié)中發(fā)揮關鍵作用，參與固有免疫和適應性免疫過程。研究機制主要聚焦于其對T細胞和NK細胞的激活、分化與功能調控，以及在炎癥和自身免疫性疾病發(fā)生發(fā)展中的作用，是免疫相關疾病治療研究熱點。試劑盒檢測范圍為3.9 pg/ml-250 pg/ml，靈敏度為0.975 pg/ml?？蓮V泛應用于免疫學基礎研究，例如評估炎癥性疾病模型中的細胞因子釋放水平、監(jiān)測體外免疫細胞刺激后的IL-12分泌動態(tài)，或探究腫瘤微環(huán)境中免疫調節(jié)機制?？蒲腥藛T可通過定量分析不同實驗條件下IL-12的表達變化，為感染性疾病、自身免疫疾病及癌癥免疫治療相關研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    IL-12/P70
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates
  • 檢測范圍：
    3.9 pg/ml-250 pg/ml
  • 靈敏度：
    0.975 pg/ml
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    104

    Range %

    97-111

    1:2

    Average %

    92

    Range %

    85-99

    1:4

    Average %

    96

    Range %

    90-101

    1:8

    Average %

    90

    Range %

    83-97

  • 回收率：

    The recovery of human IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    98

    91-104

    EDTA plasma (n=4)

    92

    85-99

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    250

    2.333

    2.434

    2.383

    2.196

    125

    1.229

    1.297

    1.263

    1.075

    62.5

    0.853

    0.828

    0.840

    0.652

    31.25

    0.571

    0.559

    0.565

    0.377

    15.6

    0.345

    0.358

    0.351

    0.164

    7.8

    0.287

    0.273

    0.280

    0.092

    3.9

    0.240

    0.238

    0.239

    0.051

    0

    0.191

    0.185

    0.188

    　

  • 本試劑盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human IL-12 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled IL-12 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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