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  4. Pig Interleukin 12,IL-12/P70 ELISA Kit

Pig Interleukin 12,IL-12/P70 ELISA Kit

  • 中文名稱：
    豬白介素12(IL-12/P70)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13288p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬白介素12(IL-12/P70)酶聯(lián)免疫試劑盒（CSB-E13288p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IL12/P70含量。IL12/P70是一種重要的靶點(diǎn)。它在免疫系統(tǒng)中發(fā)揮關(guān)鍵作用，能夠調(diào)節(jié)免疫細(xì)胞功能，參與機(jī)體炎癥反應(yīng)和免疫應(yīng)答過程。相關(guān)研究主要聚焦于其信號(hào)傳導(dǎo)通路和與疾病的關(guān)聯(lián)機(jī)制，有望為炎癥性疾病、腫瘤等的治療提供新策略。試劑盒檢測(cè)范圍為0.45 ng/mL-30 ng/mL，靈敏度為0.11 ng/mL。適用于豬類動(dòng)物模型中的免疫調(diào)控機(jī)制研究、疫苗效力評(píng)估、感染性疾病或炎癥反應(yīng)模型中細(xì)胞因子網(wǎng)絡(luò)的動(dòng)態(tài)分析；適用于畜牧獸醫(yī)研究、免疫毒理學(xué)實(shí)驗(yàn)及生物制劑開發(fā)等領(lǐng)域的定量分析需求本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL12A ELISA Kit; Interleukin-12 subunit alpha ELISA Kit; IL-12A ELISA Kit; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit; CLMF p35 ELISA Kit; IL-12 subunit p35 ELISA Kit
  • 縮寫：
    IL-12/P70
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    0.45 ng/mL-30 ng/mL
  • 靈敏度：
    0.11 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %88-96
    1:2Average %96
    Range %91-101
    1:4Average %84
    Range %80-89
    1:8Average %93
    Range %88-97
  • 回收率：
    The recovery of pig IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9086-95
    EDTA plasma (n=4)9490-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    302.549 2.452 2.501 2.331
    151.789 1.761 1.775 1.605
    7.51.025 1.004 1.015 0.845
    3.750.591 0.549 0.570 0.400
    1.80.402 0.374 0.388 0.218
    0.90.321 0.309 0.315 0.145
    0.450.258 0.245 0.252 0.082
    00.173 0.167 0.170 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-6 superfamily
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: ssc:397053

    STRING: 9823.ENSSSCP00000012501

    UniGene: Ssc.13