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  4. Rat Interleukin 12,IL-12/P70 ELISA KIT

Rat Interleukin 12,IL-12/P70 ELISA KIT

  • 中文名稱：
    大鼠白介素12(IL-12/P70)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07364r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠白介素12(IL-12/P70)酶聯(lián)免疫試劑盒（CSB-E07364r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL12/P70含量。IL12/P70是重要細(xì)胞因子，在免疫調(diào)節(jié)中發(fā)揮關(guān)鍵作用。它能激活T細(xì)胞和NK細(xì)胞來(lái)增強(qiáng)免疫應(yīng)答。研究圍繞其信號(hào)通路、與疾病關(guān)聯(lián)機(jī)制展開(kāi)，旨在通過(guò)調(diào)節(jié)它的活性開(kāi)發(fā)治療感染、腫瘤及自身免疫疾病的新療法。試劑盒檢測(cè)范圍為3.12 pg/mL-200 pg/mL，該產(chǎn)品適用于科研場(chǎng)景中免疫機(jī)制研究，如炎癥性疾病模型評(píng)估、疫苗免疫效果分析或藥物干預(yù)后IL - 12/P70的動(dòng)態(tài)變化觀測(cè)，其中血清/血漿樣本可用于系統(tǒng)性免疫狀態(tài)分析，組織勻漿則適合局部炎癥微環(huán)境研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    N/A
  • 縮寫：
    IL-12/P70
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    3.12 pg/mL-200 pg/mL
  • 靈敏度：
    0.78 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %96
    Range %91-106
    1:2Average %94
    Range %91-98
    1:4Average %93
    Range %89-96
    1:8Average %87
    Range %82-96
  • 回收率：
    The recovery of rat IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)10398-107
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.557 2.656 2.607 2.425
    1002.023 2.122 2.073 1.891
    501.448 1.468 1.458 1.276
    250.928 0.917 0.923 0.741
    12.50.578 0.569 0.574 0.392
    6.250.415 0.405 0.410 0.228
    3.120.303 0.308 0.306 0.124
    00.181 0.182 0.182 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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