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  4. Bovine Alpha-2-macroglobulin(A2M) ELISA kit

Bovine Alpha-2-macroglobulin(A2M) ELISA kit

  • 中文名稱：
    牛α2巨球蛋白(A2M)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL001004BO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產品詳情

  • 產品描述：
    牛α2巨球蛋白(A2M)酶聯(lián)免疫試劑盒（CSB-EL001004BO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的A2M含量。A2M即α?-巨球蛋白，是血漿中一種多功能糖蛋白。它能抑制多種蛋白水解酶活性，參與細胞的生長、修復等過程。在研究機制上，主要關注其對蛋白酶的捕獲作用、與細胞表面受體的相互作用，以及在疾病發(fā)生發(fā)展中所扮演的角色。試劑盒檢測范圍為0.312 μg/mL- 20 μg/mL，適用于科研場景中牛類動物模型的炎癥機制研究、免疫調控分析或蛋白酶活性相關疾病模型的分子機制探索本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    A2MAlpha-2-macroglobulin ELISA kit; Alpha-2-M ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 μg/mL- 20 μg/mL
  • 靈敏度：
    0.078 μg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine A2M in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %98
    Range %90-103
    1:200Average %90
    Range %82-94
    1:400Average %101
    Range %95-106
    1:800Average %108
    Range %101-112
  • 回收率：
    The recovery of bovine A2M spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8882-91
    EDTA plasma (n=4)9387-99
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/mlOD1OD2Average
    200.127 0.131 0.129
    100.248 0.233 0.241
    50.490 0.523 0.507
    2.50.909 0.911 0.910
    1.251.439 1.464 1.452
    0.6251.853 1.789 1.821
    0.3122.460 2.400 2.430
    02.795 2.702 2.749
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • 基因功能參考文獻：
    1. Mutations in the promoter region and the 3'-untranslated region of the A2M gene are involved in the mastitis susceptibility in dairy cows. PMID: 25237709
    2. We report here for the first time the direct isolation of Spp24 covalently bound to alpha2-macroglobulins as a core complex (Spp24-alpha2M) from bovine calf serum. PMID: 22949401
    3. Exon 29 c.3535A>T in the alpha-2-macroglobulin gene causing aberrant splice variants is associated with mastitis. PMID: 22923050
    4. These results suggest the possible involvement of cathepsin E in disruption of the structural and functional integrity of alpha 2-macroglobulin in the endolysosome system. PMID: 12631277
    5. Alpha2-macroglobulin is a substrate and an endogenous inhibitor for ADAMTS-4 and ADAMTS-5 PMID: 14715656
    6. Amounts of alpha(2)-M receptor in granulosa cells were strongly inversely associated with concentrations of estradiol in dominant and subordinate follicles. PMID: 15001551

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Protease inhibitor I39 (alpha-2-macroglobulin) family
  • 組織特異性：
    Plasma.
  • 數據庫鏈接：