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Bovine Beta-Hydroxybutyric Acid,β-OHB ELISA Kit

  • 中文名稱:
    牛β羥丁酸(βOHB)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E10056b
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    牛β羥丁酸(βOHB)酶聯(lián)免疫試劑盒(CSB-E10056b)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿樣本中的β-OHB含量。β-OHB即β-羥基丁酸,是酮體的主要成分之一。它在人體代謝中發(fā)揮著重要作用,可作為能量底物供能。研究發(fā)現(xiàn)其能通過(guò)調(diào)節(jié)多種信號(hào)通路,參與細(xì)胞代謝、炎癥反應(yīng)、氧化應(yīng)激等過(guò)程,在神經(jīng)保護(hù)、代謝性疾病等方面研究較多。試劑盒檢測(cè)范圍為50 nmol/mL-800 nmol/mL,適用于畜牧科研領(lǐng)域,例如探究牛群能量代謝特征、營(yíng)養(yǎng)調(diào)控對(duì)酮體生成的影響,或作為代謝綜合征模型的生物標(biāo)志物檢測(cè)工具。實(shí)驗(yàn)操作兼容常規(guī)實(shí)驗(yàn)室設(shè)備,為動(dòng)物營(yíng)養(yǎng)學(xué)、獸醫(yī)學(xué)研究提供高效、穩(wěn)定的檢測(cè)方案,數(shù)據(jù)結(jié)果僅用于科學(xué)研究,不涉及其他用途。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名:
    beta-Hydroxybutyric acid, beta-OHB
  • 縮寫(xiě):
    β-OHB
  • 種屬:
    Bos taurus (Bovine)
  • 樣本類型:
    serum, plasma
  • 檢測(cè)范圍:
    50 nmol/mL-800 nmol/mL
  • 靈敏度:
    9.5 nmol/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine β-OHB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 88-95  
    1:2 Average % 96  
    Range % 91-103  
    1:4 Average % 98  
    Range % 92-104  
    1:8 Average % 95  
    Range % 89-100  
  • 回收率:
    The recovery of bovine β-OHB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 90-103  
    EDTA plasma (n=4) 95 90-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    nmol/ml OD1 OD2 Average    
    800 0.136 0.138 0.137    
    400 0.309 0.306 0.308    
    200 0.674 0.682 0.678    
    100 1.470 1.475 1.473    
    50 2.706 2.712 2.709    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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