Bovine Fibroblast Growth Factor 23 (FGF23) ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

牛成纖維細胞生長因子23(FGF23)酶聯(lián)免疫試劑盒（CSB-E16193B）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的FGF23含量。FGF23即成纖維細胞生長因子 23，主要由骨細胞分泌。它能調(diào)節(jié)磷代謝和維生素 D 水平，與多種疾病相關(guān)，如慢性腎臟病礦物質(zhì)和骨異常。研究機制集中于其信號通路，包括與 klotho 蛋白相互作用及下游信號傳導(dǎo)等。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于科研領(lǐng)域探究牛磷代謝機制、骨骼疾病模型構(gòu)建、腎臟功能研究及相關(guān)營養(yǎng)調(diào)控實驗，為畜牧獸醫(yī)研究、動物營養(yǎng)學及基礎(chǔ)醫(yī)學領(lǐng)域提供可靠的檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

FGF23
Uniprot No.：

E1BJU2
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine FGF23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	86-99
1:2	Average %	103
	Range %	97-107
1:4	Average %	91
	Range %	81-94
1:8	Average %	100
	Range %	90-105

回收率：

The recovery of bovine FGF23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	94-108
EDTA plasma (n=4)	89	84-94

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.