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  4. Bovine Growth/differentiation factor 8(MSTN) ELISA kit

Bovine Growth/differentiation factor 8(MSTN) ELISA kit

  • 中文名稱：
    牛生長分化因子8(MSTN)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL015057BO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛生長分化因子8(MSTN)酶聯(lián)免疫試劑盒（CSB-EL015057BO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的MSTN含量。MSTN 即肌肉生長抑制素，是調控肌肉發(fā)育的關鍵負性因子。其背景在于它的正常表達能維持肌肉量平衡。研究機制方面，它可抑制肌肉干細胞增殖分化，減少成肌細胞生成及肌纖維生長。對其深入研究有望為肌肉萎縮等疾病治療帶來突破。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，廣泛應用于畜牧科學領域，包括動物肌肉生長調控研究、遺傳育種效果評估、營養(yǎng)干預實驗以及肌肉相關疾病模型的分子機制探索，為科研人員提供可靠的定量分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    MSTN ELISA kit; GDF8 ELISA kit; MHGrowth/differentiation factor 8 ELISA kit; GDF-8 ELISA kit; Myostatin ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    6.25 ng/mL-400 ng/mL
  • 靈敏度：
    1.56 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine MSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 88
    Range % 82-93
    1:2 Average % 97
    Range % 90-101
    1:4 Average % 98
    Range % 88-103
    1:8 Average % 104
    Range % 97-109
  • 回收率：
    The recovery of bovine MSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 99 91-105
    EDTA plasma (n=4) 89 81-94
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    400 1.954 1.902 1.928 1.805
    200 1.534 1.487 1.511 1.388
    100 1.004 1.056 1.030 0.907
    50 0.652 0.672 0.662 0.539
    25 0.416 0.405 0.411 0.288
    12.5 0.285 0.273 0.279 0.156
    6.25 0.217 0.212 0.215 0.092
    0 0.125 0.121 0.123 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Acts specifically as a negative regulator of skeletal muscle growth.
  • 基因功能參考文獻：
    1. GH/HpaII locus as candidate marker for body weight in cattle rather than MSTN/DraI. PMID: 29023020
    2. Data indicate that the the promoter trap vector PIII-myostatin could knock out the bovine myostatin gene. PMID: 25867424
    3. The effects of myostatin and myogenic factor 5 polymorphisms on growth and muscle traits of Marchigiana breed were assessed. PMID: 25023801
    4. we demonstrate zygote injection of TALEN mRNA can also produce gene-edited cattle and sheep. In both species we have targeted the myostatin (MSTN) gene. PMID: 25204701
    5. proof-of-concept study is the first to produce MSTN mutations in cattle, and may allow the development of genetically modified strains of double-muscled cattle. PMID: 24743319
    6. Mutations in the leader peptide of the bovine myostatin gene effectively promote the proliferation of bovine fibroblast cells. PMID: 24583167
    7. there were 18 SNPs identified in the Qinchuan cattle promoter region compared with those of other cattle compared to the Red Angus cattle myostatin promoter region. PMID: 24065681
    8. A 3-way interaction of myostatin genotype (MG), season, and trigonometric function periodicities of 24 h and 12 h indicate that a genotype x environment interaction exists for MG. PMID: 23572263
    9. These results show for the first time that myostatin regulates the differential expression of chemokines in skeletal muscle cells. PMID: 23838214
    10. bovine myostatin is a specific target of miR-27b and that miRNAs contribute to explain additive phenotypic hypertrophy in Piedmontese cattle selected for the MSTN gene mutation PMID: 23510267
    11. Mutations in the myostatin gene, responsible for the double muscling condition in cattle, were targeted to estimate the time since the most recent common ancestor. Each myostatin allele had a recent common ancestor (<400 years ago). PMID: 22497537
    12. MSTN promoter polymorphism g.-371T>A may affect carcass traits. PMID: 21743995
    13. Expressions of C-EBPalpha and myostatin in muscles were higher in the concentrate-fed group than in the grass hay-fed group. PMID: 21641731
    14. The sequence of the bovine myostatin gene promoter and first intron from Qinchuan and Red Angus cattle, was analyzed. PMID: 21125331
    15. the selection of the myostatin gene "+" allele has the potential to increase conventional milk production traits in the dual-purpose Belgian Blue breed. PMID: 21700058
    16. The I442M mutation in the NCAPG gene and the Q204X mutation in the GDF8 gene, were identified as substantial modulators of pre- and/or postnatal growth in cattle. PMID: 20647382
    17. extracellular matrix molecules may modulate myostatin activity PMID: 20163680
    18. mutations Q204X and nt821 affect carcass and meat quality PMID: 19966162
    19. Northern blot analysis revealed no appreciable change in myostatin mRNA levels between proliferating myoblasts and differentiated myotubes. PMID: 15975431
    20. Results suggest that myostatin auto-regulates its gene expression through a Smad7 dependent mechanism in myogenic cells. PMID: 16110474
    21. myostatin loss-of-function mutation affected several physiological processes involved in the development and determination of the functional characteristics of muscle tissue PMID: 17331240
    22. F94L genotype can produce an intermediate, non-double muscling phenotype, which should be of significant value for beef cattle producers. PMID: 17617213
    23. The results provide strong evidence that this myostatin F94L variant provides an intermediate and more useful phenotype than the more severe double-muscling phenotype caused by knockout mutations in the myostatin gene. PMID: 18245504
    24. Single-nucleotide polymorphism in the myostatin gene 5'-flanking region is associated with abnormal skeletal muscle growth and development. PMID: 18670060
    25. 11-bp deletion in the bovine myostatin was found at a low frequency (0.04) with no animals homozygous for the mutation in the sample population (536 animals)in a commercial population of Scottish Aberdeen Angus beef cattle. PMID: 18822096
    26. findings suggest that the endogenous myostatin preferentially down-regulates the expression of the fast 2x MyHC and participates in differentiation of myofiber types during early bovine myogenesis PMID: 18941308
    27. The objective of this study was to quantify the effects of the Charolais-specific inactive myostatin allele on phenotypic means and genetic parameters of heifer breeding traits. PMID: 19213706
    28. The gene expression pattern of inhibitory proteins related to MSTN signaling suggests a strong downregulation of this pathway in response to prolonged stretching. PMID: 19705480
    29. DNA sequencing revealed three, seven and four polymorphisms in exons 1, 2 and 3, respectively of the myostatin gene. PMID: 19731204

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  • 相關疾病：
    Defects in MSTN are the cause of the double-muscle phenotype or muscular hypertrophy (mh), an autosomal recessive disease frequently found in the Belgian blue and Piedmontese cattle breeds. This disease is characterized by an increased number of muscle fibers (hyperplasia), resulting in an increase in muscle mass of 20-25%.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    TGF-beta family
  • 組織特異性：
    Specifically expressed in developing and adult skeletal muscle. Highest levels found in the hindlimb semimembranosus and biceps-femoris muscles; low levels in other hindlimb muscles.
  • 數(shù)據(jù)庫鏈接：

    KEGG: bta:281187

    STRING: 9913.ENSBTAP00000015674

    UniGene: Bt.605