Bovine Heat Shock Protein 72 (HSP-72) ELISA Kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

牛熱休克蛋白72(HSP-72)酶聯(lián)免疫試劑盒（CSB-E15831B）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HSPA2含量。HSPA2屬熱休克蛋白家族，在細胞應激反應等過程起重要作用。其背景上與細胞增殖、凋亡等密切相關。研究機制方面，它可通過調節(jié)蛋白折疊、維持蛋白穩(wěn)態(tài)等影響細胞生物學行為，在腫瘤等疾病研究中是潛在靶點。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于畜牧科研中評估熱應激對牛群的影響、疾病模型構建或環(huán)境壓力下分子調控機制研究，亦可為疫苗開發(fā)或營養(yǎng)干預實驗提供定量數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
別名：

HSPA2 ELISA Kit; HSP70-3 ELISA Kit; HSPA3 ELISA Kit; Heat shock-related 70 kDa protein 2 ELISA Kit; Heat shock 70 kDa protein 3 ELISA Kit; HSP70.3 ELISA Kit
縮寫：

HSPA2
Uniprot No.：

P34933
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine HSP-72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	87-98
1:2	Average %	97
	Range %	92-105
1:4	Average %	98
	Range %	94-108
1:8	Average %	86
	Range %	82-92

回收率：

The recovery of bovine HSP-72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-98
EDTA plasma (n=4)	92	87-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. Plays a role in spermatogenesis. In association with SHCBP1L may participate in the maintenance of spindle integrity during meiosis in male germ cells.
亞細胞定位：

Cytoplasm, cytoskeleton, spindle.
蛋白家族：

Heat shock protein 70 family
數(shù)據(jù)庫鏈接：

KEGG: bta:281827

UniGene: Bt.19759