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  4. Bovine Heparin-binding growth factor 2(FGF2) ELISA kit

Bovine Heparin-binding growth factor 2(FGF2) ELISA kit

  • 中文名稱：
    牛堿性成纖維細(xì)胞生長因子/肝素結(jié)合生長因子2(FGF2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL008625BO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛堿性成纖維細(xì)胞生長因子/肝素結(jié)合生長因子2(FGF2)酶聯(lián)免疫試劑盒（CSB-EL008625BO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FGF2含量。FGF2（成纖維細(xì)胞生長因子2）是成纖維細(xì)胞生長因子家族的重要成員，具有促進(jìn)細(xì)胞生長、分化和血管生成等功能。其研究機(jī)制涉及通過結(jié)合FGFR受體激活細(xì)胞內(nèi)信號通路，如RAS-MAPK、PI3K-Akt等，參與細(xì)胞增殖、分化、遷移和血管生成等生理過程。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于科研領(lǐng)域中對FGF2功能機(jī)制的研究，包括但不限于心血管疾病模型、腫瘤微環(huán)境分析、創(chuàng)傷愈合過程等研究方向，可為細(xì)胞培養(yǎng)上清液、體液樣本及組織提取物中的FGF2動態(tài)變化提供可靠檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    FGF2Fibroblast growth factor 2 ELISA Kit; FGF-2 ELISA Kit; Basic fibroblast growth factor ELISA Kit; bFGF ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; HBGF-2) [Cleaved into: Kidney-derived growth factor] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine FGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 92
    Range % 87-98
    1:2 Average % 97
    Range % 93-102
    1:4 Average % 84
    Range % 81-89
    1:8 Average % 88
    Range % 83-95
  • 回收率：
    The recovery of bovine FGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 101 98-104
    EDTA plasma (n=4) 92 89-97
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    400 2.082 2.212 2.147 1.991
    200 1.298 1.302 1.300 1.144
    100 0.826 0.885 0.856 0.700
    50 0.527 0.564 0.546 0.390
    25 0.341 0.344 0.343 0.187
    12.5 0.288 0.298 0.293 0.137
    6.25 0.229 0.232 0.231 0.075
    0 0.152 0.160 0.156 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation.
  • 亞細(xì)胞定位：
    Secreted. Nucleus.
  • 蛋白家族：
    Heparin-binding growth factors family
  • 數(shù)據(jù)庫鏈接：