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  4. Bovine Inhibin beta A chain(INHBA) ELISA kit

Bovine Inhibin beta A chain(INHBA) ELISA kit

  • 中文名稱：
    牛抑制素-βA鏈(INHBA)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL011719BO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產品詳情

  • 產品描述：
    牛抑制素-βA鏈(INHBA)酶聯(lián)免疫試劑盒（CSB-EL011719BO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的INHBA含量。INHBA是一種靶點。它在細胞生長、分化等生理過程中或發(fā)揮重要作用。研究機制上，聚焦于其在信號通路的作用，探索其與疾病發(fā)生發(fā)展關聯(lián)。深入了解它，有助于開發(fā)針對該靶點的藥物，為相關疾病治療帶來新思路。試劑盒檢測范圍為15 pg/mL-600 pg/mL，適用于畜牧業(yè)中繁殖性能評估、生殖系統(tǒng)疾病模型研究，以及實驗室中對INHBA在組織表達水平、激素調控網絡的相關機制探索。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
  • 別名：
    INHBAInhibin beta A chain ELISA Kit; Activin beta-A chain ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15 pg/mL-600 pg/mL
  • 靈敏度：
    15 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine INHBA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 91-98  
    1:2 Average % 94  
    Range % 86-103  
    1:4 Average % 92  
    Range % 89-98  
    1:8 Average % 96  
    Range % 91-102  
  • 回收率：
    The recovery of bovine INHBA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 92-100  
    EDTA plasma (n=4) 96 91-103  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average 　  
    600 0.161 0.159 0.160 　  
    300 0.371 0.353 0.362 　  
    138 0.586 0.555 0.571 　  
    45 0.867 0.874 0.871 　  
    15 1.489 1.492 1.491 　  
    0 2.368 2.441 2.405 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
  • 基因功能參考文獻：
    1. Results confirm the prodevelopmental actions of activin A and indicate that CTGF may also function as an embryokine by regulating the number of ICM cells in the blastocyst and altering gene expression. Low concentrations of HGF were inhibitory to development. PMID: 27185102
    2. The expression of the components of the activin-inhibin-follistatin system is altered in bovine cystic ovarian disease. PMID: 25001504
    3. Results support a physiological role for granulosa-derived inhibin as an antagonist of bone morphogenetic protein action on thecal androgen synthesis. PMID: 20739376
    4. activin AB is a lignd for activin type IB receptor ALK7 and is responsible for activin-mediated secretion of insulin from pancreatic beta cell line, MIN6. PMID: 15196700
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    TGF-beta family
  • 數(shù)據(jù)庫鏈接：