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  4. Bovine Insulin-like growth factor II(IGF2) ELISA kit

Bovine Insulin-like growth factor II(IGF2) ELISA kit

  • 中文名稱：
    牛胰島素樣生長因子II(IGF2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL011088BO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛胰島素樣生長因子II(IGF2)酶聯(lián)免疫試劑盒（CSB-EL011088BO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的IGF2含量。IGF2即胰島素樣生長因子 2，在胚胎發(fā)育、細胞增殖與分化等過程中發(fā)揮關鍵作用。其異常表達與多種腫瘤發(fā)生發(fā)展相關。研究聚焦于其信號通路機制，以及與其他因子相互作用，旨在探索以 IGF2 為靶點的腫瘤診斷和治療新策略。試劑盒檢測范圍為31.25 ng/mL-2000 ng/mL，該產(chǎn)品適用于畜牧科研中生長調控機制研究、營養(yǎng)代謝評估，以及基礎醫(yī)學領域對IGF2生物學功能的探索，例如在牛胚胎發(fā)育模型、肌肉生長研究或代謝綜合征動物模型中的應用。其寬泛的檢測范圍尤其適合動態(tài)監(jiān)測IGF2水平變化，為深入解析該因子在生理及病理過程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IGF2Insulin-like growth factor II ELISA Kit; IGF-II ELISA Kit; Erythrotropin) [Cleaved into: Insulin-like growth factor II; Preptin] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    31.25 ng/mL-2000 ng/mL
  • 靈敏度：
    7.81 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 107
    Range % 100-114
    1:2 Average % 103
    Range % 101-106
    1:4 Average % 87
    Range % 84-91
    1:8 Average % 94
    Range % 90-96
  • 回收率：
    The recovery of bovine IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 90-100
    EDTA plasma (n=4) 88 85-91
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    2000 1.958 1.952 1.955 1.858
    1000 1.166 1.076 1.121 1.024
    500 0.671 0.654 0.663 0.566
    250 0.398 0.389 0.394 0.297
    125 0.263 0.254 0.259 0.162
    62.5 0.177 0.169 0.173 0.076
    31.25 0.135 0.129 0.132 0.035
    0 0.096 0.097 0.097 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF2 is influenced by placental lactogen. Also involved in tissue differentiation. In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver. Acts as a ligand for integrin which is required for IGF2 signaling. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation. Inhibits myoblast differentiation and modulates metabolism via increasing the mitochondrial respiration rate.; Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
  • 基因功能參考文獻：
    1. Methylation pattern in a CpG island of the IGF2 gene in cumulus cells from 1-3 mm and >/= 8.0 mm follicles and the effects of in vitro maturation on this pattern. PMID: 24174298
    2. Our results provide evidence that polymorphisms in the IGF2 gene are associated with growth traits, and may be used for marker-assisted selection in beef cattle breeding program PMID: 24374893
    3. The identification of the polymorphisms in the IGF2 gene that were significantly associated with growth traits in cattle. PMID: 23957672
    4. Polymorphisms in the IGF2 gene are associated with cattle growth traits. PMID: 23900197
    5. The LEP, IGF2 and CCL2 genes showed allelic expression imbalance in liver, kidney and pituitary glands of Polish Holstein-Friesian bulls. PMID: 23184004
    6. Sex-sorting procedure by flow cytometry did not affect the overall DNA methylation patterns of the IGF2 and IGF2R genes, although individual variation in their methylation patterns among bulls was observed. PMID: 22128039
    7. The reduction in IGFs mRNA level after 5 days of life in the duodenum (IGF-1 and IGF-2) and in the jejunum (IGF-1) was associated with reduction in villi length (duodenum and jejunum) and the increase of crypt depth (duodenum). PMID: 22439332
    8. Changes in the methylation pattern of IGF2 during in vitro maturation were different between incompetent and competent oocytes, and this characteristic may be useful as a molecular marker in studies of oocyte competence in cattle. PMID: 20833870
    9. Data describes the long-term changes to skeletal muscle growth and IGF1, 2, 1R, and 2R mRNA expression in progeny after exposure to high and low levels of maternal nutrient intake during the first two trimesters of gestation in the bovine. PMID: 21056085
    10. findings support work which suggests that the insulin-like growth factor 2 locus is an important biological regulator of milk production in dairy cattle PMID: 20822563
    11. effects of insulin, IGF-I and IGF-II on apoptosis and cell proliferation in bovine blastocysts in vitro PMID: 12112582
    12. IGF2 is subjected to extensive transcriptional regulation through multiple promoters, alternative splicing and polyadenylation, as well as genetic imprinting.IGF2 regulation is age-, tissue-, promoter-, and allele-specific PMID: 16120826
    13. Identification of a previously unknown differentially methylated region in exon 10 of the bovine IGF2 gene. PMID: 16644179
    14. Indel polymorphism associated with breeding value in bulls. PMID: 17242995
    15. The role of insulin-like growth factor 2 (IGF2) and the regulation of the IGF2 receptor (IGF2R) during follicular development were studied. PMID: 17360960
    16. Embryonic tissues from NT-derived embryos had higher expression of IGF-II mRNA than in vitro production embryonic tissues. PMID: 17628655
    17. The effect of single nucleotide polymorphisms in 6 genes and their associations with production factors in beef cattle are reported. PMID: 17785604
    18. study to assess the mRNA expression of IGF-I, IGF-II, IGF-IR and IGF-IIR in bovine oocytes and different stages of preimplantation embryos PMID: 19013732
    19. Dats detected that SNPs of IGF2 gene has close relationship with carcass and meat quality traits in Qinchuan cattle PMID: 19073573
    20. Hypomethylation trends in the intergenic region of the imprinted IGF2 and H19 genes in cloned cattle. PMID: 19282114
    21. Among embryos developing in vivo, the level of DNA methylation in the IGF2 intragenic DMR was significantly lower in female than in male blastocysts PMID: 19341790

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 數(shù)據(jù)庫鏈接：