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  4. Bovine Interferon α,IFN-α ELISA Kit

Bovine Interferon α,IFN-α ELISA Kit

  • 中文名稱：
    牛α干擾素(IFN-α)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13726B
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛α干擾素(IFN-α)酶聯(lián)免疫試劑盒（CSB-E13726B）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的IFN-α含量。IFN-α即α-干擾素，是機體受病毒感染等刺激后，由白細胞產(chǎn)生的細胞因子。其具有抗病毒、抗腫瘤和免疫調(diào)節(jié)等作用。研究機制主要是與細胞表面受體結(jié)合，激活信號通路，誘導(dǎo)多種抗病毒蛋白產(chǎn)生，抑制病毒復(fù)制并調(diào)節(jié)免疫細胞活性。試劑盒檢測范圍為25 pg/mL-1000 pg/mL，適用于畜牧獸醫(yī)研究、抗病毒藥物開發(fā)等科研領(lǐng)域，如牛病毒性腹瀉（BVDV）、口蹄疫等病毒感染模型的免疫機制研究，或疫苗免疫后干擾素水平動態(tài)監(jiān)測等實驗，為探索IFN-α在抗病毒免疫中的功能提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Interferon alpha-1 ELISA Kit
  • 縮寫：
    IFN-α
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    25 pg/mL-1000 pg/mL
  • 靈敏度：
    25 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 91-101  
    1:2 Average % 91  
    Range % 85-98  
    1:4 Average % 95  
    Range % 90-103  
    1:8 Average % 96  
    Range % 90-102  
  • 回收率：
    The recovery of bovine IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-98  
    EDTA plasma (n=4) 97 91-103  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1000 0.156 0.149 0.153    
    500 0.362 0.336 0.349    
    230 0.561 0.541 0.551    
    75 0.865 0.856 0.861    
    25 1.542 1.508 1.525    
    0 2.390 2.356 2.373    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Alpha/beta interferon family
  • 數(shù)據(jù)庫鏈接：