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  4. Bovine Interleukin 1,IL-1 ELISA Kit

Bovine Interleukin 1,IL-1 ELISA Kit

  • 中文名稱(chēng)：
    牛白介素1(IL-1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E12019B
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛白介素1(IL-1)酶聯(lián)免疫試劑盒（CSB-E12019B）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL1A含量。IL1A是重要靶點(diǎn)。它屬白細(xì)胞介素-1家族，在免疫和炎癥反應(yīng)中起關(guān)鍵作用。研究機(jī)制方面，聚焦于其信號(hào)通路，了解它如何與受體結(jié)合、激活下游因子從而引發(fā)炎性反應(yīng)，以此探尋針對(duì)性干預(yù)手段，為治療相關(guān)疾病提供方向。試劑盒檢測(cè)范圍為250 pg/mL-4000 pg/mL，適用于畜牧科研中免疫機(jī)制探索、疫苗效力評(píng)估或炎癥模型構(gòu)建等研究場(chǎng)景，為牛類(lèi)動(dòng)物免疫相關(guān)研究提供標(biāo)準(zhǔn)化工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
  • 縮寫(xiě)：
    IL-1
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    250 pg/mL-4000 pg/mL
  • 靈敏度：
    250 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 85-94  
    1:2 Average % 92  
    Range % 87-97  
    1:4 Average % 84  
    Range % 80-88  
    1:8 Average % 98  
    Range % 90-102  
  • 回收率：
    The recovery of bovine IL-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 89 83-96  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    4000 0.147 0.141 0.144    
    2000 0.296 0.279 0.288    
    1000 0.431 0.467 0.449    
    500 0.782 0.823 0.803    
    250 1.862 1.826 1.844    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • 基因功能參考文獻(xiàn)：
    1. These findings suggest that the elevated levels of IL-1alpha found in the osteoarthritis environment heighten fibroblast-like synoviocytes sensitivity to fluid shear by altering both intercellular communication and individual cell sensitivity, which could affect downstream functions and contribute to progression of the disease state PMID: 28716465
    2. Protein kinase R plays a pivotal role in oncostatin M and interleukin-1 signalling in bovine articular cartilage chondrocytes. PMID: 22287113
    3. Testicular IL-1 alpha and IL-1 beta concentrations were highest in the early post-natal period; however, IL-1 bioactivity and IL-6 concentrations were greatest in the immediate pre-pubertal period. PMID: 19144032
    4. Genes for IL-1alpha and IL-1beta are expressed and a functional IL-1R is present in bovine corpora lutea throughout luteal phase. IL-1alpha and IL-1beta may have different roles as regulating PGF(2alpha) and PGE(2) production during luteal phase. PMID: 15253926
    5. non-metalloproteinase mechanisms participate in IL-1-induced matrix degradation and loss of tissue material properties PMID: 17174540
    6. These results suggest that IL-1alpha and IL-1beta are produced by the stromal cells, that IL-1beta is produced by the epithelial cells, and that IL-1alpha is a more potent stimulator of prostaglandin and plasminogen activator in bovine endometrial cells. PMID: 18930361

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  • 亞細(xì)胞定位：
    Cytoplasm. Secreted.
  • 蛋白家族：
    IL-1 family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: bta:281250

    STRING: 9913.ENSBTAP00000013665

    UniGene: Bt.191