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  4. Bovine Interleukin 10,IL-10 ELISA Kit

Bovine Interleukin 10,IL-10 ELISA Kit

  • 中文名稱：
    牛白介素10，IL-10酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12917B
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛白介素10，IL-10酶聯(lián)免疫試劑盒（CSB-E12917B）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL10含量。IL10，即白細(xì)胞介素-10，是一種具有抗炎和免疫調(diào)節(jié)功能的細(xì)胞因子。它主要由抗原遞呈細(xì)胞如活化的T細(xì)胞、單核細(xì)胞、B細(xì)胞和巨噬細(xì)胞分泌。IL10通過與其受體結(jié)合，激活JAK1和TYK2激酶，進(jìn)而調(diào)節(jié)STAT1和STAT3信號通路，抑制Th1細(xì)胞因子產(chǎn)生，并抑制抗原呈遞細(xì)胞的細(xì)胞因子產(chǎn)生能力，發(fā)揮其抗炎作用。試劑盒檢測范圍為5 pg/mL-1000 pg/mL，適用于實驗室研究牛類炎癥相關(guān)疾病機制、疫苗免疫效果評估或畜牧領(lǐng)域健康監(jiān)測等科研用途本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    5 pg/mL-1000 pg/mL
  • 靈敏度：
    2.5 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 89-98  
    1:2 Average % 95  
    Range % 91-98  
    1:4 Average % 96  
    Range % 87-102  
    1:8 Average % 97  
    Range % 91-104  
  • 回收率：
    The recovery of bovine IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 95-100  
    EDTA plasma (n=4) 96 92-103  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1000 0.098 0.095 0.097    
    250 0.301 0.285 0.293    
    80 0.487 0.458 0.473    
    20 0.972 0.975 0.974    
    5 1.364 1.316 1.340    
    0 2.128 2.211 2.170    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

靶點詳情

  • 功能：
    Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
  • 基因功能參考文獻(xiàn)：
    1. Studied expression of Interleukin 10 (IL-10) in blood and milk samples of 25 healthy and 25 mastitic cows. Found IL-10 expression to be significantly higher in the blood and milk samples of mastitic cows compared to the healthy ones. PMID: 28267404
    2. This study seems to indicate that PGE2 in cattle does not produce an anti-inflammatory effect by increasing the synthesis of IL-10. PMID: 25286662
    3. IL-10 mRNA expression increased on day 8 in the mononuclear leukocytes of pregnant cows. In a cell culture experiment, interferon-tau stimulated stimulated IL-10 mRNA expression in mononuclear leukocytes. PMID: 22052007
    4. Bovine IL-10 potently inhibits the activation of human myeloid cells in response to toll like receptor activation. PMID: 21464967
    5. Levels in milk are altered in the presence of bacteria. PMID: 15372997
    6. Monocytes obtained from cows with subclinical infection with MAP had upregulated expression of IL-10 and SOCS-3, which may have attenuated the capacity of mononuclear phagocytes to initiate inflammatory and adaptive immune responses. PMID: 16111147

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-10 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: bta:281246

    STRING: 9913.ENSBTAP00000008793

    UniGene: Bt.4723