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  4. Bovine Interleukin-18(IL18) ELISA kit

Bovine Interleukin-18(IL18) ELISA kit

  • 中文名稱：
    牛白介素18(IL18)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL011608BO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛白介素18(IL18)酶聯(lián)免疫試劑盒（CSB-EL011608BO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL18含量。IL-18是一種促炎細(xì)胞因子，在先天性和適應(yīng)性免疫反應(yīng)中發(fā)揮關(guān)鍵作用。它可誘導(dǎo)Th1細(xì)胞產(chǎn)生IFN-γ，參與免疫調(diào)節(jié)。多個(gè)研究表明，IL-18在多種炎癥性疾病、自身免疫病和腫瘤的發(fā)病機(jī)制中起重要作用，是極具潛力的治療靶點(diǎn)。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，適用于研究牛感染性疾病模型（如病毒或細(xì)菌感染）、免疫調(diào)節(jié)機(jī)制探索、疫苗效力評(píng)估等科研場景，也可用于評(píng)估營養(yǎng)干預(yù)或環(huán)境應(yīng)激對(duì)牛免疫狀態(tài)的影響。通過標(biāo)準(zhǔn)化操作流程，研究者可快速獲得IL18在免疫相關(guān)研究中的動(dòng)態(tài)變化數(shù)據(jù)，為深入解析牛類免疫系統(tǒng)功能提供技術(shù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL18 ELISA Kit; IGIF ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 88
    Range % 83-92
    1:2 Average % 97
    Range % 91-105
    1:4 Average % 95
    Range % 92-99
    1:8 Average % 93
    Range % 87-98
  • 回收率：
    The recovery of bovine IL18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 90-110
    EDTA plasma (n=4) 97 90-105
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 3.011 2.978 2.995 2.900
    1000 2.511 2.522 2.517 2.422
    500 1.496 1.504 1.500 1.405
    250 0.705 0.716 0.711 0.616
    125 0.382 0.375 0.379 0.284
    62.5 0.226 0.234 0.230 0.135
    31.25 0.152 0.159 0.156 0.061
    0 0.093 0.097 0.095 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells.
  • 基因功能參考文獻(xiàn)：
    1. These results suggest that BAPC-1 is a stem/progenitor cell line and modulates the immuno-endocrine function of the anterior pituitary cells through its production of interleukin-18 and the IL-18 receptor. PMID: 18761716
  • 亞細(xì)胞定位：
    Cytoplasm. Secreted.
  • 蛋白家族：
    IL-1 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: bta:281249

    STRING: 9913.ENSBTAP00000055793

    UniGene: Bt.234