Bovine Interleukin 2,IL-2 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (7) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

牛白介素2(IL-2)酶聯(lián)免疫試劑盒（CSB-E09810b）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL2含量。IL2是重要的細(xì)胞因子，可調(diào)節(jié)免疫細(xì)胞生長、增殖和分化。它在免疫系統(tǒng)激活和維持免疫平衡方面作用關(guān)鍵。其研究機制主要圍繞調(diào)控T細(xì)胞和NK細(xì)胞功能，在腫瘤免疫治療、自身免疫性疾病等領(lǐng)域是研究熱門靶點。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于科研領(lǐng)域探究牛類免疫機制、疫苗效力評估、畜牧病原感染模型中的細(xì)胞免疫應(yīng)答分析，以及炎癥性疾病或免疫抑制相關(guān)研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IL2 ELISA kit; IL-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
縮寫：

IL2
Uniprot No.：

P05016
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-90
1:2	Average %	100
	Range %	97-103
1:4	Average %	101
	Range %	98-105
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of bovine IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	96	92-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
基因功能參考文獻(xiàn)：
1. Pretreatment of neonatal PBMC with IL-1beta, TNF-alpha or IFN-gamma promotes mitogenic response to ConA through up-regulating the production of IL-2 and the expression of the mature IL-2 receptor. PMID: 12906107
亞細(xì)胞定位：

Secreted.
蛋白家族：

IL-2 family
數(shù)據(jù)庫鏈接：

KEGG: bta:280822

STRING: 9913.ENSBTAP00000027819

UniGene: Bt.341