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  4. Bovine Interleukin 6,IL-6 ELISA Kit

Bovine Interleukin 6,IL-6 ELISA Kit

  • 中文名稱：
    牛白介素6(IL-6)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12899B
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛白介素6(IL-6)酶聯(lián)免疫試劑盒（CSB-E12899B）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL6含量。IL-6（白介素6）是一種多功能細胞因子，由多種細胞類型產(chǎn)生，如單核巨噬細胞、成纖維細胞、T淋巴細胞等。它通過與其受體結(jié)合，參與調(diào)節(jié)免疫反應(yīng)、急性期反應(yīng)和造血功能。IL-6在多種疾病的發(fā)生發(fā)展中扮演重要角色，如炎癥、自身免疫疾病和癌癥。其研究機制涉及IL-6與受體的相互作用、信號傳導(dǎo)途徑（如JAK2/STAT3）的激活，以及其在細胞增殖、分化和遷移中的作用。試劑盒檢測范圍為5 pg/mL-1000 pg/mL，適用于畜牧領(lǐng)域炎癥性疾病研究、疫苗效力評估、抗感染免疫機制探索等科研場景，也可用于動物源性生物制品（如血清或細胞培養(yǎng)上清）的質(zhì)量控制。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL6 Interleukin-6 ELISA Kit; IL-6 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    5 pg/mL-1000 pg/mL
  • 靈敏度：
    2.5 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 

  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 90-97  
    1:2 Average % 95  
    Range % 91-99  
    1:4 Average % 95  
    Range % 88-101  
    1:8 Average % 95  
    Range % 90-100  
  • 回收率：
    The recovery of bovine IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 94-101  
    EDTA plasma (n=4) 96 92-103  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1000 0.071 0.075 0.073    
    250 0.192 0.205 0.199    
    80 0.421 0.451 0.436    
    20 0.876 0.897 0.887    
    5 1.345 1.316 1.331    
    0 2.116 2.215 2.166    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

靶點詳情

  • 最新研究進展：
    IL6是一種白細胞介素，具有調(diào)節(jié)免疫反應(yīng)和炎癥反應(yīng)的作用。最新研究表明，IL6在心血管疾病、惡性腫瘤和神經(jīng)系統(tǒng)疾病等多種疾病的發(fā)生和發(fā)展中發(fā)揮重要作用。研究還發(fā)現(xiàn)，針對IL6的治療方法在臨床實踐中取得了很好的療效，可以有效減輕疾病的癥狀和改善患者的生活質(zhì)量。
  • 功能：
    Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.; IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury. In the adaptive immune response, is required for the differentiation of B cells into immunoglobulin-secreting cells. Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Required to drive naive CD4(+) T cells to the Th17 lineage. Also required for proliferation of myeloma cells and the survival of plasmablast cells.; Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF. Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance. 'Trans-signaling' in central nervous system also regulates energy and glucose homeostasis. Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand. Also acts as a myokine. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection. Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration.
  • 基因功能參考文獻：
    1. STA3 facilitates TLR4-dependent IL-6 and IL-8 production via IL-6 receptor-positive feedback in endometrial cells. PMID: 26813342
    2. when a confluent endometrial epithelial cell barrier is faced with infection and damage, chemokines attract immune cells to the uterine lumen, but IL6 is solely secreted apically to ensure immune cells are only exposed to IL6 once they reach the lumen. PMID: 25740541
    3. The results revealed that the peak expression of IL6 and 21 was on DPV 28 which correlated well with the FMDV antibody titer and plummeted to the prevaccination titer level by 60 DPV. PMID: 24555796
    4. Exposure to follicular fluid transiently increased the transcript levels of IL8 and PTGS2, and decreased the expression of SOD2, GPX3, DAB2, and NR3C1. TNF and IL6 levels were also decreased while those of NAMPT were unaffected. PMID: 24186266
    5. Testicular IL-1 alpha and IL-1 beta concentrations were highest in the early post-natal period; however, IL-1 bioactivity and IL-6 concentrations were greatest in the immediate pre-pubertal period. PMID: 19144032
    6. The results showed that the expression of TNF-alpha, iNOS, and IL-6 in alveolar macrophages was up-regulated by stimulation with the recombinant Mce4A protein of M. bovis; in contrast, expression of IL-12 was unaffected.[IL-6, IL-12] PMID: 17530193
    7. results show for the first time that interleukin-6 (IL6), in the presence of its soluble receptor (sIL-6R), induces activation of JAK1, JAK2, and STAT1/STAT3 proteins in bovine articular chondrocytes. PMID: 12419823
    8. Mechanical injury potentiates the catabolic effects of TNFalpha and IL-6/sIL-6R in causing proteoglycan degradation in human and bovine cartilage. PMID: 19790045

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    IL-6 superfamily
  • 數(shù)據(jù)庫鏈接：

    KEGG: bta:280826

    STRING: 9913.ENSBTAP00000019865

    UniGene: Bt.3686