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  4. Bovine Lysozyme (LZM) ELISA Kit

Bovine Lysozyme (LZM) ELISA Kit

  • 中文名稱：
    牛溶菌酶(LZM)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E15829B
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛溶菌酶(LZM)酶聯(lián)免疫試劑盒（CSB-E15829B）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LZM含量。牛溶菌酶（LZM）是一種具有抗菌、抗炎、抗病毒和免疫調(diào)節(jié)功能的堿性蛋白，其作用機制主要是通過裂解細菌細胞壁的肽聚糖結(jié)構(gòu)，以及與病毒蛋白結(jié)合使其失活。近年來，研究者通過基因工程和轉(zhuǎn)基因技術(shù)，提高了LZM的表達量和活性，拓展了其在食品、醫(yī)藥和生物材料等領(lǐng)域的應(yīng)用。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，支持科研人員探究反芻動物免疫調(diào)節(jié)機制、病原體防御反應(yīng)等方向，例如在畜牧業(yè)中評估飼養(yǎng)環(huán)境對牛群免疫健康的影響，或基礎(chǔ)研究中解析LZM在組織局部抗感染過程中的動態(tài)表達規(guī)律，為動物模型構(gòu)建及生物標志物研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    LYZ1Lysozyme C ELISA Kit; EC 3.2.1.17 ELISA Kit; 1,4-beta-N-acetylmuramidase C ELISA Kit
  • 縮寫：
    LZM
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine LZM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:200 Average % 101
    Range % 96-105
    1:400 Average % 90
    Range % 85-95
    1:800 Average % 106
    Range % 100-110
    1:1600 Average % 104
    Range % 99-109
  • 回收率：
    The recovery of bovine LZM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 94 87-98
    EDTA plasma (n=4) 100 94-105
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average
    20 0.147 0.143 0.145
    10 0.187 0.195 0.191
    5 0.286 0.273 0.280
    2.5 0.404 0.399 0.402
    1.25 0.634 0.604 0.619
    0.625 0.970 0.934 0.952
    0.312 1.809 1.822 1.816
    0 2.516 2.457 2.487
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
  • 蛋白家族：
    Glycosyl hydrolase 22 family
  • 組織特異性：
    Stomach-specific.
  • 數(shù)據(jù)庫鏈接：

    KEGG: bta:280849

    UniGene: Bt.29367