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  4. Bovine Myoglobin(MB) ELISA kit

Bovine Myoglobin(MB) ELISA kit

  • 中文名稱：
    牛肌紅蛋白(MB)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL013529BO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛肌紅蛋白(MB)酶聯(lián)免疫試劑盒（CSB-EL013529BO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的MB含量。試劑盒檢測(cè)范圍為15.6 ng/mL-1000 ng/mL，常用于動(dòng)物肌肉生理研究、運(yùn)動(dòng)醫(yī)學(xué)模型構(gòu)建及肉品加工過(guò)程中肌肉品質(zhì)評(píng)估等科研領(lǐng)域。適用于探究牛肌肉疾病機(jī)制、運(yùn)動(dòng)生理學(xué)實(shí)驗(yàn)或肌肉相關(guān)生物標(biāo)志物篩選等研究，為畜牧科學(xué)、食品科學(xué)及基礎(chǔ)醫(yī)學(xué)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    MB ELISA Kit; Myoglobin ELISA Kit
  • 縮寫：
    MB
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue Homogenates
  • 檢測(cè)范圍：
    15.6 ng/mL-1000 ng/mL
  • 靈敏度：
    3.9 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 90
    Range % 83-99
    1:2 Average % 91
    Range % 89-99
    1:4 Average % 95
    Range % 90-100
    1:8 Average % 95
    Range % 86-98
  • 回收率：
    The recovery of bovine MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 87-99
    EDTA plasma (n=4) 95 90-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    1000 2.184 2.201 2.193 2.099
    500 1.628 1.643 1.636 1.542
    250 1.023 1.015 1.019 0.925
    125 0.565 0.575 0.570 0.476
    62.5 0.318 0.305 0.312 0.218
    31.2 0.179 0.192 0.186 0.092
    15.6 0.129 0.133 0.131 0.037
    0 0.095 0.092 0.094 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

Do you have both Beef and buffalo Myoglobin (MB) detection kit in ELISA format.Could you please discuss with your technical team for the following : 1. Cross-reactivity of Bovine MB kit for Buffalo MB. I am awaiting your response.

A:
Hi
Bovine Myoglobin(MB) ELISA kit,#CSB-EL013529BO This kit is specific to general bovine.We only test ordinary bovine.But there should be no big problems on Beef and buffalo.We suggest you do pre-testing first and see
Q:

Could you please revisit/discuss once more with your technical team for the following : 1. Coating Antibody is polyclonal - Yes or No. 2. Biotin Labeled antibody is polyclonal - Yes or No.
I am awaiting your response.

A:
Bovine Myoglobin(MB) ELISA kit,#CSB-EL013529BO This kit is specific to general bovine.We did not valiadate this kit on Buffalo.But there should be no big problems.We suggest you do pre-testing first and see

靶點(diǎn)詳情

  • 功能：
    Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
  • 基因功能參考文獻(xiàn)：
    1. These results suggest an important role of genetics in meat color variation for cattle raised under the tropic conditions. PMID: 26946475
    2. Glycine at E14 in myoglobin enhances autoxidation and hemin loss rates. PMID: 22681513
  • 蛋白家族：
    Globin family
  • 數(shù)據(jù)庫(kù)鏈接：