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  4. Bovine Parathyroid hormone(PTH) ELISA kit

Bovine Parathyroid hormone(PTH) ELISA kit

  • 中文名稱：
    牛甲狀旁腺素(PTH)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL018987BO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛甲狀旁腺素(PTH)酶聯(lián)免疫試劑盒（CSB-EL018987BO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PTH含量。甲狀旁腺激素（PTH）是甲狀旁腺主細胞分泌的肽類激素。它對鈣磷代謝具有關(guān)鍵調(diào)節(jié)作用。研究機制方面，主要通過作用于骨和腎臟，促進骨鈣釋放、增加腎小管對鈣的重吸收，維持血鈣水平穩(wěn)定，且在骨代謝相關(guān)疾病研究中受關(guān)注。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，該產(chǎn)品適用于科研場景中牛PTH相關(guān)機制研究，如鈣代謝調(diào)控、骨骼發(fā)育分析、營養(yǎng)代謝評估及內(nèi)分泌疾病模型構(gòu)建等領(lǐng)域，為畜牧養(yǎng)殖、動物生理學(xué)及基礎(chǔ)醫(yī)學(xué)研究提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    PTH ELISA Kit; Parathyroid hormone ELISA Kit; PTH ELISA Kit; Parathyrin ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 91
    Range % 86-99
    1:2 Average % 96
    Range % 90-110
    1:4 Average % 90
    Range % 83-99
    1:8 Average % 95
    Range % 85-105
  • 回收率：
    The recovery of bovine PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 92 88-98
    EDTA plasma (n=4) 96 90-110
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 2.626 2.603 2.615 2.498
    1000 2.365 2.325 2.345 2.228
    500 1.986 1.992 1.989 1.872
    250 1.502 1.486 1.494 1.377
    125 0.841 0.835 0.838 0.721
    62.5 0.486 0.474 0.480 0.363
    31.25 0.371 0.375 0.373 0.256
    0 0.115 0.119 0.117 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
  • 基因功能參考文獻：
    1. These data highlight the ability of PTH to phosphorylate beta-catenin directly via PKA. PMID: 26851123
    2. These data suggest that prostaglandin E2 acting via EP4R on bone marrow macrophages committed to the osteoblast cell lineage, stimulated secretion of a factor or factors that acted to suppress PTH-stimulated osteoblast differentiation. PMID: 23639875
    3. These results suggest that in vivo PTH treatment increased in vitro osteoclastogenesis and resorption without altering the number of osteoclast precursors. PMID: 21611963
    4. Osteoblast 2-deoxyglucose uptake and glycogen synthesis were increased after exposure to low concentrations (0.1 nmol/l and above) of PTH. PMID: 21076856
    5. Parathyroid hormone stimulated growth and decreased Col-X deposition via phosphotidylinositol-3,4,5 triphosphate kinase and mitogen activating protein kinase pathways in avian sterna. PMID: 19957341
    6. likely involvement of the Sp family in regulating PTH gene expression through interactions with an Sp1 DNA element in the hormone's promoter. PMID: 12810570
    7. alternative cis-acting protein-binding elements may determine the regulation of PTH mRNA stability in response to changes in serum calcium and phosphate PMID: 15824859
    8. a possible role for the Wnt signaling pathway in PTH actions in bone PMID: 15962290
    9. Data suggest that calcium-mediated destabilization of parathyroid hormone mRNA requires gene transcription and involves increases in cytosolic Ca. PMID: 18096993

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Parathyroid hormone family
  • 數(shù)據(jù)庫鏈接：

    KEGG: bta:280903

    STRING: 9913.ENSBTAP00000025399

    UniGene: Bt.95