Bovine Pregnancy Specific Protein B(PSPB)ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

牛妊娠特異性蛋白B（PSPB）酶聯(lián)免疫試劑盒（CSB-E13353B）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PAG1含量。PAG1是重要靶點。它在細(xì)胞信號傳導(dǎo)等方面發(fā)揮作用，與細(xì)胞生長、增殖等生理過程相關(guān)。研究其機制有助于了解疾病發(fā)生發(fā)展，可能為腫瘤等疾病的治療提供新思路，通過調(diào)控PAG1有望開發(fā)出新型治療手段。試劑盒檢測范圍為0.06 ng/mL-24 ng/mL，本產(chǎn)品適用于畜牧科研中妊娠狀態(tài)監(jiān)測、胎盤功能評估、生殖內(nèi)分泌研究及動物繁殖性能分析等場景，其寬泛的樣本兼容性可滿足血清/血漿直接檢測及組織樣本經(jīng)勻漿處理后的定量需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Pregnancy-associated glycoprotein 1 ELISA Kit; PAG 1 ELISA Kit; EC 3.4.23.- ELISA Kit; Pregnancy-specific protein B ELISA Kit; PSP-B ELISA Kit
縮寫：

PAG1
Uniprot No.：

Q29432
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.06 ng/mL-24 ng/mL
靈敏度：

0.06 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine PSPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
Range %	90-104
1:2	Average %	94
Range %	88-105
1:4	Average %	90
Range %	85-96
1:8	Average %	97
Range %	89-105

回收率：

The recovery of bovine PSPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-94
EDTA plasma (n=4)	97	90-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Appears to be proteolytically inactive.
基因功能參考文獻(xiàn)：
1. This study examines expression patterns of the genes SERPINA14, PAG, and PAG2 at the fetal-maternal interface in dairy heifers experimentally infected with N caninum during the second trimester of pregnancy, when most abortions takes place in natural conditions. PMID: 27045629
2. The clinical implications of these findings are that following infection with N. caninum, most cows will experience some level of placental damage and that this injury correlates with foetal fluid PAG levels and pH. PMID: 26936628
3. These findings indicate that IFN-gamma production correlates negatively and the production of antibodies against N. caninum is uncorrelated with plasma pregnancy-associated glycoproteins levels. PMID: 24456132
4. Restraint stress for 2 h during early pregnancy in heifers increased blood cortisol, but it did not affect the concentrations of progesterone and PAG-1 between days 30 to 40 of gestation. PMID: 21872319
亞細(xì)胞定位：

Secreted, extracellular space.
蛋白家族：

Peptidase A1 family
組織特異性：

Trophoblast and placental tissue. Produced specifically in the invasive binucleate cells of the placenta. Becomes detectable in maternal serum soon after implantation.
數(shù)據(jù)庫鏈接：

KEGG: bta:281964

STRING: 9913.ENSBTAP00000025593

UniGene: Bt.15401