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  4. Bovine Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

Bovine Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

  • 中文名稱：
    牛催乳素(PRL/LTH)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14002B
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛催乳素(PRL/LTH)酶聯(lián)免疫試劑盒（CSB-E14002B）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的PRL含量。催乳素（PRL）是一種由垂體前葉分泌的多肽激素，主要促進(jìn)乳腺發(fā)育和乳汁分泌。其分泌受下丘腦-垂體軸調(diào)控，多巴胺等激素可抑制其分泌。催乳素在泌乳過程中起關(guān)鍵作用，其受體為跨膜糖蛋白，參與信號轉(zhuǎn)導(dǎo)。試劑盒檢測范圍為20 μIU/mL-8000 μIU/mL，本產(chǎn)品適用于畜牧業(yè)研究中泌乳機(jī)制探索、繁殖周期監(jiān)測、應(yīng)激水平評估等科研場景，為優(yōu)化奶牛飼養(yǎng)管理或乳腺疾病研究提供數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    20 μIU/mL-8000 μIU/mL
  • 靈敏度：
    20 μIU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 91-100  
    1:2 Average % 95  
    Range % 86-104  
    1:4 Average % 90  
    Range % 85-95  
    1:8 Average % 96  
    Range % 92-108  
  • 回收率：
    The recovery of bovine PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 91-102  
    EDTA plasma (n=4) 95 89-102  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μIU/ml OD1 OD2 Average    
    8000 0.361 0.369 0.365    
    1500 0.695 0.704 0.700 　  
    250 1.205 1.199 1.202    
    70 1.524 1.520 1.522    
    20 1.703 1.709 1.706    
    0 2.245 2.243 2.244    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Prolactin acts primarily on the mammary gland by promoting lactation.
  • 基因功能參考文獻(xiàn)：
    1. Taken together, these results support novel functions of prolactin as a modulator of the innate immune response that do not involve the classical prolactin pathway. PMID: 26341952
    2. The presence and localization of prolactin receptor are consistent with expression data reported for other species, and the presence of PIP and prolactin in seminal fluid is consistent with data generated in humans. PMID: 25533929
    3. Functionally reciprocal mutations of the prolactin signaling pathway define hairy and slick cattle. PMID: 25519203
    4. The single nucleotide polymorphism rs42646708 of cattle XKR4 was significantly associated with serum prolactin concentrations and explained 2.45% of the phenotypic variation. PMID: 24666329
    5. Five mutations were identified in exonic region and eleven in associated intronic regions in PRL gene in 100 cattle from four Pakistani cattle breeds. PMID: 24065524
    6. this study identifies a biochemical mechanism for the regulation of SCFAs on bovine GH and PRL gene transcription in dairy cow anterior pituitary cells PMID: 24177567
    7. These data suggest that PRL protects brain endothelial cells against methamphetamine-induced toxicity PMID: 23988027
    8. Bovine prolactin improved the expression of human transferrin through such a possible mechanism that bovine prolactin activated STAT5a transcription expression. PMID: 22829284
    9. There was no significant difference between 1134 locus and milk performance traits of 5'-UTR of PRL gene PMID: 22207382
    10. Staphylococcus aureus infection inhibits nuclear factor kappa B activation mediated by prolactin in bovine mammary epithelial cells. PMID: 21843629
    11. Study of genetic variation in Yakutian cattle (Bos taurus L.) using the prolactin bPRL, growth hormone bGH, and transcription factor bPit-1 genes PMID: 20391788
    12. This is the second study reporting single nucleotide polymorphisms in the 5'-regulatory region of PRL gene, which interfere with milk production traits. PMID: 19714484
    13. evidence for a functional coupling between the PRL salt bridge and phosphorylation site indicates that either in vivo phosphorylation or specific mutations that destabilize the salt bridge impair biological activity. PMID: 12850287
    14. determination of the effects of exposure to different lengths of daylight during the dry period on circulating PRL and PRL receptor mRNA expression in lymphocytes and mammary tissue during the transition to lactation PMID: 15591374
    15. Expression of PRL and prolactin receptor (PRLR) mRNA is demonstrated for the first time in bovine corpus luteum throughout the luteal phase. PMID: 16435374
    16. The A-->G transition at position -1043 abolishes the recognition site for Hsp92II restriction endonuclease. PMID: 17929163
    17. Prolactin in ovarian follicular fluid stimulates endothelial cell proliferation. PMID: 19672107

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 數(shù)據(jù)庫鏈接：

    KEGG: bta:280901

    STRING: 9913.ENSBTAP00000020313

    UniGene: Bt.7197