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  4. Bovine Selenoprotein P(SEPP1) ELISA kit

Bovine Selenoprotein P(SEPP1) ELISA kit

  • 中文名稱：
    牛硒蛋白P(SEPP1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL021018BO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛硒蛋白P(SEPP1)酶聯(lián)免疫試劑盒（CSB-EL021018BO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SEPP1含量。SEPP1即硒蛋白P，在人體硒穩(wěn)態(tài)維持和抗氧化防御等方面發(fā)揮重要作用。其研究機(jī)制涉及運(yùn)輸硒到各組織，還參與細(xì)胞內(nèi)的抗氧化過程，減輕氧化應(yīng)激。當(dāng)前圍繞它在神經(jīng)系統(tǒng)、心血管系統(tǒng)疾病及癌癥等方面的作用也有諸多研究。試劑盒檢測范圍為10 ng/mL-160 ng/mL，該產(chǎn)品可廣泛應(yīng)用于動物營養(yǎng)學(xué)研究中的硒元素吸收評估、氧化應(yīng)激相關(guān)疾病模型的生物標(biāo)志物分析，或環(huán)境毒理學(xué)中重金屬暴露對硒蛋白代謝影響的探索，為畜牧健康管理及基礎(chǔ)醫(yī)學(xué)研究提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    SELENOP ELISA Kit; Selenoprotein P ELISA Kit; SeP ELISA Kit; Selenoprotein P-like protein ELISA Kit
  • 縮寫：
    SEPP1
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    10 ng/mL-160 ng/mL
  • 靈敏度：
    5 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine SEPP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:100 Average % 89
    Range % 86-102
    1:200 Average % 97
    Range % 91-105
    1:400 Average % 93
    Range % 88-100
    1:800 Average % 91
    Range % 87-96
  • 回收率：
    The recovery of bovine SEPP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 92 86-99
    EDTA plasma (n=4) 93 85-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average 　
    160 0.105 0.102 0.104 　
    80 0.196 0.189 0.193 　
    40 0.321 0.332 0.327 　
    20 0.622 0.608 0.615 　
    10 1.098 1.108 1.103 　
    0 2.193 2.216 2.205 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Constitutes a major selenium pool in the brain and may play an important role in developing and/or modulating the morphology of neurons and/or glial cells.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Selenoprotein P family
  • 組織特異性：
    Brain and kidney. Most prominently expressed in the cerebellar cortex, hippocampus and olfactory bulb.
  • 數(shù)據(jù)庫鏈接：

    KEGG: bta:282066

    UniGene: Bt.64610